Callegari C, Lami F, Cornia G L, Pironi L, Cocchi M, Turchetto E, Barbara L
JPEN J Parenter Enteral Nutr. 1985 May-Jun;9(3):334-8. doi: 10.1177/0148607185009003334.
To establish the effect of chemically defined formula diets on pancreatic mass, 58 male Wistar rats, weighing approximately 200 g, were fed orally one of the following diets for 20 days: standard diet; partially hydrolyzed diet; elemental diet. The diets were isocaloric and provided 73 cal/day per rat. At the end of the experiment, the rats were killed and pancreas removed to assess wet weight and DNA and RNA content. Compared to the controls, the elemental diet caused a reduction in pancreatic wet weight (p less than 0.005), pancreatic DNA (p less than 0.001), and RNA content (p less than 0.001). In the group fed partially hydrolyzed diet, only pancreatic DNA content showed an highly significant reduction when compared with the reference group (p less than 0.001), whereas the value of RNA failed to reach the statistical significance. We found an increase of the ratio mg RNA/mg DNA in the partially hydrolyzed and elemental diet groups. These results suggest that long-term nutrition with the partially hydrolyzed diet, and more strongly with the elemental diet, may induce pancreatic hypoplasia.
为确定化学成分明确的配方饮食对胰腺质量的影响，将58只体重约200克的雄性Wistar大鼠分为三组，每组分别经口喂食以下一种饮食，持续20天：标准饮食；部分水解饮食；要素饮食。这些饮食的热量相同，每只大鼠每天提供73卡路里。实验结束时，处死大鼠并取出胰腺，以评估其湿重以及DNA和RNA含量。与对照组相比，要素饮食导致胰腺湿重减少（p<0.005）、胰腺DNA减少（p<0.001）以及RNA含量减少（p<0.001）。在喂食部分水解饮食的组中，与参照组相比，仅胰腺DNA含量显示出极显著降低（p<0.001），而RNA值未达到统计学显著性。我们发现，部分水解饮食组和要素饮食组的mg RNA/mg DNA比值增加。这些结果表明，长期食用部分水解饮食，尤其是要素饮食，可能会诱发胰腺发育不全。
