Mandal Shreya, Patra Arpita, Samanta Animesh, Roy Suchismita, Mandal Arpita, Mahapatra Tapasi Das, Pradhan Shrabani, Das Koushik, Nandi Dilip Kumar
Department of Nutrition, Microbiology and Human Physiology, Raja N L Khan Women's College, Midnapore, Pin-721102, West Bengal, India.
Asian Pac J Trop Biomed. 2013 Dec;3(12):960-6. doi: 10.1016/S2221-1691(13)60186-0.
To investigate phytochemical screening, antimicrobial activity and qualitative thin layer chromatographic separation of flavonoid components, antioxidant activity and total flavonoid compound of Terminalia arjuna.
For phytochemical screening, some common and available standard tests were done. Antimicrobial bioassay was done through agar well diffusion method. Detection of antioxidant activity and flavonoid compounds were done through thin layer chromatography. Total antioxidant activity was measured by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) in colorimetric method. Aluminum chloride colorimetric method was used for total flavonoid determination.
Phytochemical screening showed the active compounds presence in high concentration, such as phytosterol, lactones, flavonoids, phenolic compounds and tannins and glycosides. The antimicrobial activity of extract showed that greater inhibition zone against Gram negative bacteria than Gram positive bacteria. This methanolic extract showed a promising antioxidant activity, as absorption of DPPH redicles decreased in DPPH free radical scavenging assay. Flavonoids components having antioxidant property present in the methanol extract at a level of 199.00 mg quercetin equivalent/g of dried methanol extract in colorimetric method.
The Terminalia arjuna bark extract revealed the presence of bio-active constituents which are known to exhibit medicinal as well as physiological activities.
研究诃子的植物化学筛选、抗菌活性、黄酮类成分的定性薄层色谱分离、抗氧化活性及总黄酮化合物。
进行植物化学筛选时，采用了一些常见且可行的标准试验。抗菌生物测定通过琼脂孔扩散法进行。抗氧化活性和黄酮类化合物的检测通过薄层色谱法进行。总抗氧化活性采用2,2-二苯基-1-苦基肼（DPPH）比色法测定。总黄酮测定采用氯化铝比色法。
植物化学筛选表明，植物甾醇、内酯、黄酮类、酚类化合物、单宁和糖苷等高浓度活性化合物存在。提取物的抗菌活性表明，对革兰氏阴性菌的抑制圈大于革兰氏阳性菌。在DPPH自由基清除试验中，该甲醇提取物表现出有前景的抗氧化活性，因为DPPH自由基的吸收减少。比色法测定甲醇提取物中具有抗氧化特性的黄酮类成分含量为199.00毫克槲皮素当量/克干燥甲醇提取物。
诃子树皮提取物显示出存在已知具有药用和生理活性的生物活性成分。
