Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Mater Sci Eng C Mater Biol Appl. 2013 Dec 1;33(8):4633-9. doi: 10.1016/j.msec.2013.07.022. Epub 2013 Jul 24.
Osteoblasts or stem cells have been delivered into injectable calcium phosphate cement (CPC) to improve its effectiveness and biological function. However, the osteogenic potential of the new construct in vivo has been rarely reported, and there are no reports on alginate-chitosan microencapsulated osteoblasts mixed with CPC. This study aimed to develop alginate-chitosan microencapsulated mouse osteoblast MC3T3-E1 cells (AC-cells), evaluate the osteogenic potential of a calcium phosphate cement complex with these AC-cells (CPC-AC-cell), and trace the implanted MC3T3-E1 cells in vivo. MC3T3-E1 cells were embedded in alginate microcapsules, cultured in osteogenic medium for 7 days, and then covered with chitosan before mixing with a paste of β-tricalcium phosphate/calcium phosphate cement (β-TCP/CPC). The construct was injected into the dorsal subcutaneous area of nude mice. Lamellar-bone-like mineralization, newly formed collagen and angiogenesis were observed at 4 weeks. At 8 weeks, areas of newly formed collagen expanded; further absorption of β-TCP/CPC and osteoid-like structures could be seen. Cell tracing in vivo showed that implanted MC3T3-E1 cells were clearly visible at 2 weeks. These in vivo results indicate that the novel injectable CPC-AC-cell construct is promising for bone tissue engineering applications.
成骨细胞或干细胞已被递送至可注射的磷酸钙水泥 (CPC) 中，以提高其有效性和生物功能。然而，新构建体在体内的成骨潜力很少有报道，也没有关于藻酸盐-壳聚糖微囊化成骨细胞与 CPC 混合的报道。本研究旨在开发藻酸盐-壳聚糖微囊化小鼠成骨细胞 MC3T3-E1 细胞（AC 细胞），评估含有这些 AC 细胞的磷酸钙水泥复合物（CPC-AC 细胞）的成骨潜力，并在体内追踪植入的 MC3T3-E1 细胞。将 MC3T3-E1 细胞嵌入藻酸盐微胶囊中，在成骨培养基中培养 7 天，然后用壳聚糖覆盖，然后与 β-磷酸三钙/磷酸钙水泥（β-TCP/CPC）的糊状物混合。将构建体注射到裸鼠的背部皮下区域。在 4 周时观察到板层状骨样矿化、新形成的胶原蛋白和血管生成。在 8 周时，新形成的胶原蛋白区域扩大；可以看到进一步吸收的 β-TCP/CPC 和类骨质样结构。体内细胞追踪显示，植入的 MC3T3-E1 细胞在 2 周时清晰可见。这些体内结果表明，新型可注射 CPC-AC 细胞构建体有望用于骨组织工程应用。
