Department of Neurology and BNI-ASU Center for Preclinical Imaging, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, AZ 85013, United States.
J Neuroimmunol. 2013 Nov 15;264(1-2):35-40. doi: 10.1016/j.jneuroim.2013.09.008. Epub 2013 Sep 18.
Research tools are urgently needed to elucidate the specificities of NMO and MS due to their clinical similarity at the early stage of the diseases. Herein, using high-field-strength MRI we characterized the optic nerve and spinal cord lesions in 2D2(tg) mice (MOG 35-55 specific TCR). Specifically, early Blood-brain Barrier breakdown was observed in 86% of the 2D2(tg) mice, while the majority of mice showed little to no brain lesions. Further, immunohistology showed inflammatory infiltrates and demyelination in the brain and spinal cord that mirrored sites of MRI lesions, along with a decrease in AQP4 protein at lesion sites. Collectively, 2D2(tg) mice develop optic and spinal cord lesions that can be visualized by high-field rodent MRI and verified by pathological assessment. The similarity of these lesions with those seen in NMO patients suggests that the 2D2(tg) mouse might serve as a model for NMO research.
研究工具迫切需要阐明 NMO 和 MS 的特异性，因为它们在疾病的早期阶段具有相似的临床特征。在这里，我们使用高场强 MRI 对 2D2(tg) 小鼠（MOG 35-55 特异性 TCR）的视神经和脊髓病变进行了特征描述。具体来说，在 86%的 2D2(tg) 小鼠中观察到早期血脑屏障破坏，而大多数小鼠的脑部病变很少或没有。此外，免疫组织化学显示，在大脑和脊髓中存在炎症浸润和脱髓鞘，与 MRI 病变部位相吻合，同时在病变部位 AQP4 蛋白减少。综上所述，2D2(tg) 小鼠可发展为视神经和脊髓病变，可通过高场强啮齿动物 MRI 进行可视化，并通过病理评估进行验证。这些病变与 NMO 患者所见的病变相似，表明 2D2(tg) 小鼠可能成为 NMO 研究的模型。
