Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Int J Syst Evol Microbiol. 2014 Feb;64(Pt 2):456-461. doi: 10.1099/ijs.0.055350-0. Epub 2013 Oct 4.
A novel, Gram-staining-positive, facultatively anaerobic, non-motile and coccus-shaped bacterium, strain WL80(T), was isolated from the gut of an abalone, Haliotis discus hannai, collected from the northern coast of Jeju in Korea. Optimal growth occurred at 30 °C, pH 7-8 and with 1% (w/v) NaCl. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that strain WL80(T) fell within the cluster of the genus Actinomyces, with highest sequence similarity to the type strains of Actinomyces radicidentis (98.8% similarity) and Actinomyces urogenitalis (97.0% similarity). The major cellular fatty acids were C18 : 1ω9c and C16 : 0. Menaquinone-10 (H4) was the major respiratory quinone. The genomic DNA G+C content of the isolate was 70.4 mol%. DNA-DNA hybridization values with closely related strains indicated less than 7.6% genomic relatedness. The results of physiological, biochemical, chemotaxonomic and genotypic analyses indicated that strain WL80(T) represents a novel species of the genus Actinomyces, for which the name Actinomyces haliotis sp. nov. is proposed. The type strain is WL80(T) ( = KACC 17211(T) = JCM 18848(T)).
一株新型的革兰氏阳性、兼性厌氧、非运动、球菌状细菌，菌株 WL80(T)，从韩国济州岛北部海岸采集的鲍鱼 Haliotis discus hannai 的肠道中分离得到。最适生长温度为 30°C，pH 值为 7-8，NaCl 浓度为 1%（w/v）。基于 16S rRNA 基因序列的系统发育分析表明，菌株 WL80(T)属于放线菌属，与 Actinomyces radicidentis（相似度为 98.8%）和 Actinomyces urogenitalis（相似度为 97.0%）的模式菌株最为相似。主要细胞脂肪酸为 C18:1ω9c 和 C16:0。主要呼吸醌为menaquinone-10 (H4)。该分离株的基因组 DNA G+C 含量为 70.4 mol%。与亲缘关系较近的菌株的 DNA-DNA 杂交值表明基因组相关性小于 7.6%。生理、生化、化学分类和基因型分析的结果表明，菌株 WL80(T)代表放线菌属的一个新型种，命名为 Actinomyces haliotis sp. nov.。模式菌株为 WL80(T)（=KACC 17211(T)=JCM 18848(T)）。
