Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Korea.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4626-4632. doi: 10.1099/ijs.0.053892-0. Epub 2013 Aug 9.
A novel Gram-negative, aerobic, non-motile and rod-shaped bacterium, designated strain WM67(T), was isolated from the gut of an abalone (Haliotis discus hannai) collected from the northern coast of Jeju Island in Korea. Phylogenetic analyses based on the 16S rRNA gene sequence indicated that strain WM67(T) clustered in the genus Pseudoruegeria, and the highest sequence similarity was shared with Pseudoruegeria lutimaris (98.0 % similarity to the type strain). Optimal growth of the isolate occurred at 30 °C, pH 7-8 and with 1 % (w/v) NaCl. The major cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0. Ubiquinone Q-10 was the major respiratory quinone. The polar lipids of strain WM67(T) comprised phosphatidylserine, phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, an unidentified glycolipid and three unidentified lipids. The genomic DNA G+C content was 66.5 mol%. DNA-DNA hybridization indicated <17 % genomic relatedness to other members of the genus Pseudoruegeria. The physiological, biochemical, chemotaxonomic and genotypic analyses indicated that strain WM67(T) represents a novel species of Pseudoruegeria, for which the name Pseudoruegeria haliotis sp. nov. is proposed. The type strain is WM67(T) ( = KACC 17214(T) = JCM 18872(T)).
一株新的革兰氏阴性、需氧、非运动和杆状细菌，命名为 WM67(T) 菌株，从韩国济州岛北部海岸采集的鲍鱼肠道中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，WM67(T) 菌株聚类在拟魨形菌属中，与魨形菌属 Lutimaris 的相似度最高（与模式菌株的相似度为 98.0%）。该分离株的最佳生长条件为 30°C、pH7-8 和 1%（w/v）NaCl。主要细胞脂肪酸为特征脂肪酸 8（C18：1ω7c 和/或 C18：1ω6c）和 C16：0。主要呼吸醌为泛醌 Q-10。WM67(T) 菌株的极性脂由磷脂酰丝氨酸、磷脂酰胆碱、磷脂酰甘油、磷脂酰乙醇胺、一种未鉴定的糖脂和三种未鉴定的脂组成。基因组 DNA G+C 含量为 66.5mol%。DNA-DNA 杂交表明，与拟魨形菌属的其他成员的基因组相关性<17%。生理、生化、化学分类和基因型分析表明，WM67(T) 菌株代表拟魨形菌属的一个新种，建议将其命名为魨形菌属鲍鱼亚种（Pseudoruegeria haliotis sp. nov.）。模式菌株为 WM67(T)（=KACC 17214(T)=JCM 18872(T)）。
