National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, China.
J Pharmacol Sci. 2013;123(2):195-8. doi: 10.1254/jphs.13057sc. Epub 2013 Oct 4.
We investigated the anticonvulsant effect of acute Fuzi total alkaloid (FTA) in seizure induced by the GABAA-receptor antagonist pentylenetetrazole (PTZ). FTA significantly increased the seizure latency and decreased the mortality in PTZ-treated mice. Administration of PTZ increased c-Fos expression in the hippocampus, medial prefrontal cortex, and piriform cortex; and this PTZ-induced effect was inhibited by FTA in a dose-dependent manner. Furthermore, the effects of FTA on PTZ-induced seizure and c-Fos expression were reversed by the GABAA/benzodiazepine receptor-selective antagonist flumazenil. These findings suggest that the anticonvulsant effects of FTA may be related to modulation of GABAA-benzodiazepine receptor complex.
我们研究了急性附子总生物碱（FTA）对 GABAA 受体拮抗剂戊四氮（PTZ）诱导的癫痫发作的抗惊厥作用。FTA 显著延长了 PTZ 处理的小鼠的癫痫发作潜伏期，并降低了死亡率。PTZ 处理增加了海马、内侧前额叶皮层和梨状皮层中 c-Fos 的表达；而 FTA 以剂量依赖性的方式抑制了这种 PTZ 诱导的效应。此外，FTA 对 PTZ 诱导的癫痫发作和 c-Fos 表达的作用被 GABAA/苯二氮䓬受体选择性拮抗剂氟马西尼逆转。这些发现表明，FTA 的抗惊厥作用可能与 GABAA-苯二氮䓬受体复合物的调节有关。
