Department of Plant Protection, Razi University, Kermanshah, Iran.
Mol Biol Rep. 2012 Sep;39(9):8999-9006. doi: 10.1007/s11033-012-1770-1. Epub 2012 Jun 24.
RAPD (random amplification of polymorphic DNA) was used to distinguish the genetic diversities between two genera of Chrysopa and Chrysoperla (Neuroptera, Chrysopidae). Sixty specimens were collected in different places in Kermanshah, west of Iran. The wing venation was used for identification of each type of two genera, and the gender was determined by study of external genitalia. 20 random primers were used for polymerase chain reaction. Then, the electrophoresis was used for separation of the PCR products on agarose gel. 294 bands were amplified, which 235 bands were polymorph and others (59s) determined as monomorph. The electrophoresis results showed that the primers OPA02 with 19 bands and OPA03 with 8 bands successively amplified the maximum and minimum of bands among the applied primers. The results showed that there are maximum of genetic diversity and minimum of genetic similarity between Chrysopa male (Chrysopa-M) and Chrysoperla female)Chrysoperla-F) population, in contrast, there are maximum of genetic similarity and minimum of genetic diversity between Chrysoperla-M and Chrysoperla-F, and Chrysopa-M and Chrysopa-F. There are also more genetic similarities, between males and females of Chrysopa and Chrysoperla, than between male of Chrysopa with female of Chrysoperla or vice versa.
RAPD（随机扩增多态性 DNA）被用于区分两种绿蝽属和草蛉属（脉翅目，草蛉科）的遗传多样性。60 个标本在伊朗西部克尔曼沙阿的不同地方采集。通过翅脉鉴定两种属的每种类型，通过外部生殖器研究确定性别。使用 20 个随机引物进行聚合酶链反应。然后，电泳用于在琼脂糖凝胶上分离 PCR 产物。扩增了 294 个条带，其中 235 个条带为多态性，其余（59 个）为单态性。电泳结果表明，引物 OPA02 扩增了 19 个条带，OPA03 扩增了 8 个条带，在应用的引物中依次扩增了最大和最小的条带。结果表明，在绿蝽属雄性（Chrysopa-M）和草蛉属雌性（Chrysoperla-F）种群之间存在最大的遗传多样性和最小的遗传相似性，相反，在草蛉属雄性和雌性之间存在最大的遗传相似性和最小的遗传多样性，而绿蝽属雄性和雌性之间存在最小的遗传多样性。绿蝽属和草蛉属的雄性和雌性之间也存在更多的遗传相似性，而绿蝽属雄性与草蛉属雌性之间或反之亦然的遗传相似性较少。
