Rydzyński K, Dastych J
Agents Actions. 1985 Apr;16(3-4):163-5. doi: 10.1007/BF01983128.
The aim of this study was to find the early exocytotic signs in passively sensitized rat mast cells stimulated with antigen using freeze-fracturing technique and to establish if histamine release precedes or follows exocytosis. It has been shown that histamine release was detectable as early as 5 sec, and completed in approx. 20-30 sec, after challenge with antigen. However, the earliest membrane changes were observed after 20 sec exposure to the releaser. They were manifested with fusion between granule membranes and etchable pores in the plasma membrane. Areas exhibiting depletion or aggregation of intramembrane particles have not been seen. It can be concluded, that during anaphylactic reactions histamine secretion precedes the process of granule exocytosis.
本研究的目的是利用冷冻断裂技术寻找抗原刺激下被动致敏大鼠肥大细胞早期胞吐的迹象，并确定组胺释放是先于还是后于胞吐作用。结果表明，抗原攻击后最早在5秒即可检测到组胺释放，并在约20 - 30秒内完成。然而，最早的膜变化是在接触释放剂20秒后观察到的。其表现为颗粒膜与质膜上可蚀刻孔之间的融合。未观察到膜内颗粒出现耗竭或聚集的区域。可以得出结论，在过敏反应中，组胺分泌先于颗粒胞吐过程。
