Institute of Quality Standards and Testing Technologies for Agro-products, Chinese Academy of Agricultural Sciences, Beijing, China.
PLoS One. 2013 Oct 3;8(10):e76400. doi: 10.1371/journal.pone.0076400. eCollection 2013.
"Lean meat powder" is a class of toxic chemicals that have structures similar to that of β-adrenergic receptor agonists. At least 16 chemicals from this class have been specifically banned by the 176(th) bulletin of the Chinese Department of Agriculture on breeding animals, and methods for monitoring the illicit use of β-agonists in animal feed are required. Herein, a method to quantify 20 β-agonists in feed, via analyte derivatization followed by gas chromatography-tandem mass spectrometry, has been developed. The optimized method has a good linear correlation (calibration coefficient > 0.99) between the quantitative ion peak area and the concentration of β-agonists over a large working range (0.05-1 mg/kg). The limit of detection (LOD) was 0.01 mg/kg, the recoveries for three β-agonists spikes (0.05, 0.1, and 1 µg/g) in feed ranged from 75.6 to 102.4%, repeatability ranged from 1.2 to 9.4% for all of the compounds, and intermediate precisions were lower than 13.8%. This precise, accurate method was applied to quantify 20 β-agonists in actual feed samples and represents an excellent complement to existing quantification methods.
“瘦肉精”是一类具有类似β-肾上腺素受体激动剂结构的有毒化学物质。中国农业部第 176 号公告已明令禁止该类物质中的至少 16 种物质用于养殖动物，并且需要监测动物饲料中β-激动剂的非法使用。本文建立了一种基于衍生化反应后采用气相色谱-串联质谱法同时检测饲料中 20 种β-激动剂的方法。该方法在较大的工作范围内（0.05-1mg/kg）对β-激动剂的定量离子峰面积与浓度之间具有良好的线性关系（校准系数>0.99）。该方法的检出限（LOD）为 0.01mg/kg，在饲料中添加 0.05、0.1 和 1μg/g 三种β-激动剂的回收率为 75.6%-102.4%，所有化合物的重复性为 1.2%-9.4%，中间精密度低于 13.8%。该方法准确、精密，可用于实际饲料样品中 20 种β-激动剂的定量检测，是对现有检测方法的良好补充。
