a Department of Physiologic Science , Piracicaba Dental School, University of Campinas - UNICAMP , Piracicaba , Brazil ;
Biofouling. 2013;29(10):1233-42. doi: 10.1080/08927014.2013.834050. Epub 2013 Oct 7.
The present study examined the influences of the neovestitol-vestitol (NV) containing fraction isolated from Brazilian red propolis on the development of biofilm and expression of virulence factors by Streptococcus mutans using saliva-coated surfaces of hydroxyapatite. In addition, NV was tested in a rodent model of dental caries to assess its potential effectiveness in vivo. Topical applications of NV (800 μg ml(-1)) significantly impaired the accumulation of biofilms of S. mutans by largely disrupting the synthesis of glucosyltransferase-derived exopolysaccharides and the expression of genes associated with the adaptive stress response, such as copYAZ and sloA. Of even greater impact, NV was as effective as fluoride (positive control) in reducing the development of carious lesions in vivo. NV is a promising natural anti-biofilm agent that targets essential virulence traits in S. mutans, which are associated with the formation of cariogenic biofilm and the subsequent onset of dental caries disease.
本研究使用唾液包被的羟磷灰石表面，考察了从巴西红蜂胶中分离得到的含新威替醇-威替醇（NV）的馏分对变形链球菌生物膜形成和毒力因子表达的影响。此外，还在龋齿啮齿动物模型中测试了 NV，以评估其在体内的潜在有效性。NV（800μg/ml）的局部应用显著损害了变形链球菌生物膜的积累，主要通过破坏葡糖基转移酶衍生的胞外多糖的合成和与适应应激反应相关的基因（如 copYAZ 和 sloA）的表达来破坏生物膜的积累。更有影响的是，NV 在体内减少龋齿病变的发展方面与氟化物（阳性对照）一样有效。NV 是一种有前途的天然抗生物膜剂，针对变形链球菌的关键毒力特性，这些特性与致龋生物膜的形成和随后龋齿疾病的发生有关。
