Dipartimento di Scienze Farmaceutiche e Biomediche, Università di Salerno, Via Ponte Don Melillo, 84084 Fisciano, Salerno, Italy.
J Agric Food Chem. 2011 Jun 22;59(12):6484-91. doi: 10.1021/jf201280z. Epub 2011 Jun 2.
Chemical composition of propolis depends on the specificity of the local flora at the site of collection and thus on the geographic and climatic characteristics of this place. This paper describes a comparative analysis of Cuban red propolis (CRP), Brazilian red propolis (BRP), and Dalbergia ecastophyllum exudates (DEE) by high-performance liquid chromatography with diode-array detection and tandem mass spectrometry. The aim of this study was to investigate the overall chemical profile and the botanical origin of red propolis and to suggest similarities and differences between samples collected in different tropical regions. Isoliquiritigenin (1), liquiritigenin and naringenin (2 and 17), isoflavones (3-4 and 16), isoflavans (5-7 and 18), and pterocarpans (8-13) were detected in CRP, BRP, and DEE, whereas polyisoprenylated benzophenones (PPBs) guttiferone E/xanthochymol (14a,b) and oblongifolin A (15) were detected only in BRP. Pigments responsible for the red color of DEE and red propolis were also identified as two C30 isoflavans, the new retusapurpurin B (19) and retusapurpurin A (20). PPBs and pigments were isolated and unambiguously characterized by 1D and 2D NMR analysis. These results show that red propolis samples from different tropical zones have a similar chemical composition. DEE is the main red propolis source, but the presence of PPBs in BRP suggests the contribution of different botanical sources for Brazilian samples. This chemical information is important for quality control of red propolis and its commercial products and for biological study.
蜂胶的化学成分取决于采集地当地植物群的特异性，因此也取决于该地区的地理和气候特征。本文描述了用高效液相色谱-二极管阵列检测和串联质谱法对古巴红蜂胶（CRP）、巴西红蜂胶（BRP）和 Dalbergia ecastophyllum 渗出物（DEE）进行的比较分析。本研究的目的是调查红蜂胶的整体化学特征和植物来源，并比较在不同热带地区采集的样品之间的相似性和差异性。在 CRP、BRP 和 DEE 中检测到了异甘草素（1）、甘草素和柚皮素（2 和 17）、异黄酮（3-4 和 16）、异黄烷（5-7 和 18）和紫檀烷（8-13），而多异戊二烯基苯并二氢吡喃酮（PPB）guttiferone E/xanthochymol（14a,b）和 oblongifolin A（15）仅在 BRP 中检测到。导致 DEE 和红蜂胶呈现红色的色素也被鉴定为两种 C30 异黄烷，即新的 retusapurpurin B（19）和 retusapurpurin A（20）。通过 1D 和 2D NMR 分析对 PPB 和色素进行了分离和明确的表征。这些结果表明，来自不同热带地区的红蜂胶样品具有相似的化学成分。DEE 是红蜂胶的主要来源，但 BRP 中 PPB 的存在表明巴西样品可能来自不同的植物来源。这些化学信息对于红蜂胶及其商业产品的质量控制和生物学研究非常重要。
