Zelenika D, Grima B, Pessac B
Centre de Biologie Cellulare du CNRS, Ivry/Seine, France.
J Neurochem. 1993 Apr;60(4):1574-7. doi: 10.1111/j.1471-4159.1993.tb03325.x.
A cDNA clone (MBP2) corresponding to a novel mouse myelin basic protein (MBP) mRNA has been isolated from an adult mouse bone marrow cDNA library. It contains the MBP exons 1a-7 except exon 5. Using PCR experiments we have determined that this MBP2 mRNA belongs to a new MBP mRNA family initiated upstream from exon 1b. Their 5' end extends into exon 1a and/or the region 0' previously described. These mRNAs are generated by alternative splicing of the primary transcript involving excision of exon 1a, 1b, 2, 5, or 6. Thus, these new mRNAs are produced from a promoter(s) located upstream from the major promoter 1b. They are expressed in brain (at least from embryonic day 15), in bone marrow, and in other hemolymphopoietic tissues, particularly in macrophage cells. As their expression is not restricted to myelinating cells, the function of these novel MBP mRNAs and putative proteins might not be related to myelination.
从成年小鼠骨髓cDNA文库中分离出一个与新型小鼠髓鞘碱性蛋白（MBP）mRNA对应的cDNA克隆（MBP2）。它包含MBP外显子1a - 7，但不包括外显子5。通过PCR实验我们确定，这种MBP2 mRNA属于一个新的MBP mRNA家族，该家族起始于外显子1b上游。它们的5'端延伸到外显子1a和/或先前描述的0'区域。这些mRNA是由初级转录本的可变剪接产生的，涉及外显子1a、1b、2、5或6的切除。因此，这些新的mRNA是由位于主要启动子1b上游的一个或多个启动子产生的。它们在脑（至少从胚胎第15天开始）、骨髓以及其他造血组织中表达，尤其在巨噬细胞中表达。由于它们的表达不限于髓鞘形成细胞，这些新型MBP mRNA和推定蛋白质的功能可能与髓鞘形成无关。
