Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, China; Marine Biology Institute, Shantou University, Shantou, China.
FEMS Microbiol Lett. 2013 Nov;348(2):112-9. doi: 10.1111/1574-6968.12277. Epub 2013 Oct 10.
Vibrio owensii is a potential bacterial pathogen in marine aquaculture system. In this study, five lytic phages specific against Vibrio strain B8D, closely related to V. owensii, were isolated from seawater of an abalone farm. The phages were characterized with respect to morphology, genome size, growth phenotype, as well as thermal, and pH stability. All phages were found to belong to the family Siphoviridae with long noncontractile tails and terminal fibers. Restriction analysis indicated that the five phages were dsDNA viruses with molecular weights ranging from c. 30 to 48 kb. One-step growth experiments revealed that the phages were heterogeneous in latent periods (10-70 min), rise periods (40-70 min), and burst sizes [23-331 plaque-forming units (PFU) per infected cell] at the same host strain. All phages were thermal stable and were tolerant to a wide range of pH. The results indicated that these phages could be potential candidates of a phage cocktail for biological control of V. owensii in aquaculture systems.
欧文氏弧菌是海洋水产养殖系统中的一种潜在的细菌性病原体。在本研究中，从鲍鱼养殖场的海水中分离到了 5 株专门针对与欧文氏弧菌密切相关的 B8D 菌株的裂解噬菌体。这些噬菌体在形态、基因组大小、生长表型以及热稳定性和 pH 稳定性方面的特性进行了研究。所有噬菌体均属于长尾非收缩性 Siphoviridae 科，末端带有纤维。限制性分析表明，这 5 株噬菌体均为双链 DNA 病毒，分子量在 30-48kb 之间。一步生长实验表明，在同一宿主菌株中，噬菌体潜伏期（10-70min）、上升期（40-70min）和爆发量[每个感染细胞产生的 23-331 个噬菌斑形成单位（PFU）]存在异质性。所有噬菌体均具有热稳定性，能耐受广泛的 pH 值范围。这些结果表明，这些噬菌体可能是用于水产养殖系统中控制欧文氏弧菌的噬菌体鸡尾酒的潜在候选者。
