感染食源致病性副溶血性弧菌的烈性噬菌体SHOU24的全基因组序列
Complete genome sequence of virulent bacteriophage SHOU24, which infects foodborne pathogenic Vibrio parahaemolyticus.
作者信息
Yuan Lin, Cui Zelin, Wang Yanchun, Guo Xiaokui, Zhao Yong
机构信息
College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China.
出版信息
Arch Virol. 2014 Nov;159(11):3089-93. doi: 10.1007/s00705-014-2160-x. Epub 2014 Aug 13.
A novel lytic Vibrio parahaemolyticus phage (SHOU24) belonging to the family Siphoviridae was isolated from aquatic market sewage. The phage is only able to infect V. parahaemolyticus containing a tdh gene. SHOU24 has a linear genome of 77,837 bp with a G+C content of 46.0 %. In total, 88 predicted proteins have homologues in databases, and the majority of the core genes share high sequence similarity with genes from unrelated viruses and bacteria. Genes related to lysogeny and host lysis were not detected. However, the detection method, the results of a one-step growth experiment and analysis using the Phage Classification Tool Set (PHACTS) indicate that SHOU24 is lytic. A bioinformatics analysis showed that SHOU24 is not closely related to other Vibrio phages.
从水产市场污水中分离出一种新型的溶菌性副溶血性弧菌噬菌体（SHOU24），它属于长尾噬菌体科。该噬菌体仅能感染含有tdh基因的副溶血性弧菌。SHOU24具有一个77,837 bp的线性基因组，G+C含量为46.0%。总共88个预测蛋白在数据库中有同源物，并且大多数核心基因与来自不相关病毒和细菌的基因具有高度序列相似性。未检测到与溶原性和宿主裂解相关的基因。然而，检测方法、一步生长实验结果以及使用噬菌体分类工具集（PHACTS）的分析表明SHOU24是溶菌性的。生物信息学分析表明SHOU24与其他弧菌噬菌体没有密切关系。
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