Karsten U, Papsdorf G, Roloff G, Stolley P, Abel H, Walther I, Weiss H
Eur J Cancer Clin Oncol. 1985 Jun;21(6):733-40. doi: 10.1016/0277-5379(85)90271-8.
Hybridomas producing mouse monoclonal antibodies to antigens of the human mammary carcinoma cell line, MCF-7, have been generated by electric field-mediated fusion at a frequency ten times higher than by polyethylene glycol. One of the monoclonal antibodies obtained recognizes a cytoskeletal structure restricted to epithelial cells and carcinomas with a distribution pattern resembling cytokeratin 19.
通过电场介导融合已产生了针对人乳腺癌细胞系MCF - 7抗原的产生小鼠单克隆抗体的杂交瘤，其产生频率比聚乙二醇介导的融合高十倍。所获得的一种单克隆抗体识别一种局限于上皮细胞和癌的细胞骨架结构，其分布模式类似于细胞角蛋白19。
