Currò M, Matarese G, Isola G, Caccamo D, Ventura V P, Cornelius C, Lentini M, Cordasco G, Ientile R
Department of Biomedical Sciences and Morphofunctional Imaging, AOU Policlinico "G. Martino", University of Messina, Messina, Italy.
Oral Dis. 2014 Sep;20(6):616-23. doi: 10.1111/odi.12180. Epub 2013 Sep 23.
Gingival epithelium plays a key role in the protection of oral tissues from microbial challenge, especially during the periodontal disease. This study was aimed to evaluate levels of mRNA transcripts of different forms of transglutaminase in the human gingival tissues from patients with chronic periodontitis and relative controls.
This study included 22 patients with chronic periodontitis (CP) and 22 healthy controls. For each patient, the values of probing depth (PD), clinical attachment level (CAL), and bleeding on probing (BOP) were recorded. Gene expression of transglutaminase 1, transglutaminase 2, transglutaminase 3, and metalloprotease 2 was evaluated by real-time PCR, while that of Factor XIIIA and metalloprotease 9 by RT-PCR.
The values of all the clinical parameters were significantly higher in the CP group than in the healthy control group (P < 0.05). In the CP group, the mRNA expression of transglutaminase 1 and transglutaminase 3 was significantly decreased in comparison with healthy control group. A slight nonsignificant changes of transglutaminase 2 gene expression were observed in samples from CP patients in comparison with controls.
These observations suggest that transglutaminase gene expression may be modified in response to chronic injury in the damaged gingival and emphasizes the key role of these enzymes in gingival remodelling/healing and adaptive processes.
牙龈上皮在保护口腔组织免受微生物侵害方面起着关键作用，尤其是在牙周疾病期间。本研究旨在评估慢性牙周炎患者和相对对照组的人牙龈组织中不同形式转谷氨酰胺酶的mRNA转录水平。
本研究纳入22例慢性牙周炎（CP）患者和22例健康对照者。记录每位患者的探诊深度（PD）、临床附着水平（CAL）和探诊出血（BOP）值。通过实时PCR评估转谷氨酰胺酶1、转谷氨酰胺酶2、转谷氨酰胺酶3和金属蛋白酶2的基因表达，通过逆转录PCR评估凝血因子ⅫIA和金属蛋白酶9的基因表达。
CP组所有临床参数值均显著高于健康对照组（P < 0.05）。在CP组中，与健康对照组相比，转谷氨酰胺酶1和转谷氨酰胺酶3的mRNA表达显著降低。与对照组相比，CP患者样本中转谷氨酰胺酶2基因表达有轻微的非显著性变化。
这些观察结果表明，转谷氨酰胺酶基因表达可能会因受损牙龈的慢性损伤而发生改变，并强调了这些酶在牙龈重塑/愈合和适应性过程中的关键作用。
