Elizaquível P, Aznar R, Sánchez G
Department of Microbiology and Ecology, University of Valencia, Valencia, Spain.
J Appl Microbiol. 2014 Jan;116(1):1-13. doi: 10.1111/jam.12365. Epub 2013 Nov 1.
The increase in foodborne outbreaks highlights the need for rapid, sensitive and specific methods for food safety monitoring, enabling specific detection and quantification of viable foodborne pathogens. Real-time PCR (qPCR) combined with the use of viability dyes, recently introduced, fulfils all these requirements. The strategy relies on the use of DNA-binding molecules such as propidium monoazide (PMA) or ethidium monoazide (EMA) as sample pretreatment previous to the qPCR. These molecules permeate only membrane-compromised cells and have successfully been applied for different types of foodborne pathogens, including bacteria and viruses. Moreover, those dyes have been explored to monitor different food manufacturing processes as an alternative to classical cultural methods. In this review, state-of-the-art information regarding viability PCR (v-PCR) is compiled.
食源性疾病暴发事件的增加凸显了对食品安全监测采用快速、灵敏且特异方法的需求，以便能够对存活的食源性病原体进行特异性检测和定量分析。最近引入的实时荧光定量聚合酶链反应（qPCR）结合使用活菌染料，满足了所有这些要求。该策略依赖于在qPCR之前使用诸如单叠氮丙锭（PMA）或单叠氮溴化乙锭（EMA）等DNA结合分子进行样品预处理。这些分子仅能渗透细胞膜受损的细胞，并且已成功应用于不同类型的食源性病原体，包括细菌和病毒。此外，这些染料已被用于监测不同的食品制造过程，作为传统培养方法的替代方法。在本综述中，汇编了有关活菌PCR（v-PCR）的最新信息。
