Department for Hospital Hygiene, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria.
Antimicrob Resist Infect Control. 2013 Oct 11;2(1):27. doi: 10.1186/2047-2994-2-27.
The number of bacteria recovered from a stainless steel coupon after touching a pigskin substrate with an examination glove coated on its outside with polyhexanide (PHMB), as compared to the number of bacteria recovered in the same manner with non-coated control gloves was evaluated.
Suspensions containing 1 × 109 colony-forming units of 4 clinically relevant bacterial species (Enterococcus faecium ATCC #51559; Escherichia coli ATCC #25922; Klebsiella pneumoniae ATCC #4352; and Staphylococcus aureus ATCC #33591) were used to contaminate Gamma-irradiated pigskin substrates. Bacterial recoveries from the pigskin substrate, stainless steel coupons, and each glove swatch were performed. A difference in the bacterial recovery from the stainless steel coupons after touching with coated and uncoated control gloves was measured.
For E. faecium, the coated glove showed a reduction of 4.63 log10 cfu recovery, when compared to control gloves. For E. coli, the coated glove showed 5.48 log10 cfu, for K. pneumoniae 5.03 log10 cfu, and for S. aureus 5.72 log10 cfu recovery, when compared to the non-coated control glove.
An in-vitro experiment designed to mimic cross-contamination of clinically relevant bacteria in a simulated healthcare setting following glove contact with a contaminated biological surface and cross-transfer to a stainless steel surface has demonstrated that an examination glove coated on its outside surface with PHMB was able to reduce bacterial recovery from a contaminated surface by > 4 log10 cfu, compared to a control non-coated examination glove. These elaborated results may encourage further clinical investigation on the clinical impact of an antibacterial examination glove.
将涂有聚六亚甲基双胍（PHMB）的检查手套的外侧接触涂有伽马射线的猪皮基质，与以相同方式用未涂覆的对照手套接触相比，从不锈钢卡箍上回收的细菌数量与从相同方式回收的细菌数量进行了评估。
使用含有 4 种临床相关细菌（屎肠球菌 ATCC #51559；大肠杆菌 ATCC #25922；肺炎克雷伯菌 ATCC #4352；金黄色葡萄球菌 ATCC #33591）的 1×109 个菌落形成单位的悬浮液污染伽马射线照射的猪皮基质。从猪皮基质、不锈钢卡箍和每个手套拭子中进行细菌回收。测量涂覆和未涂覆对照手套接触后从不锈钢卡箍上回收细菌的差异。
对于粪肠球菌，与对照手套相比，涂覆手套的回收率降低了 4.63 log10 cfu。对于大肠杆菌，涂覆手套的回收率为 5.48 log10 cfu，肺炎克雷伯菌为 5.03 log10 cfu，金黄色葡萄球菌为 5.72 log10 cfu，与未涂覆的对照手套相比。
一项旨在模拟临床相关细菌在模拟医疗保健环境中的交叉污染的体外实验，即在手套接触污染的生物表面后交叉转移到不锈钢表面，结果表明，与对照非涂覆的检查手套相比，涂覆有 PHMB 的检查手套的外侧表面能够减少从污染表面回收的细菌数量> 4 log10 cfu。这些详细的结果可能会鼓励进一步对具有抗菌作用的检查手套的临床影响进行临床研究。
