Pan Xu, Xu Lijing, Meng Junlong, Chang Mingchang, Cheng Yanfen, Geng Xueran, Guo Dongdong, Liu Rongzhu
College of Food Science and Engineering, Shanxi Agricultural University, Jinzhong, China.
Shanxi Key Laboratory of Edible Fungi for Loess Plateau, Taigu, China.
Front Nutr. 2022 Jun 9;9:912014. doi: 10.3389/fnut.2022.912014. eCollection 2022.
In this study, a high-efficiency and non-pollution extraction procedure, ultrasound-assisted technique with deep eutectic solvents (DESs), was applied for extraction of polysaccharides from (MIP-D). The results exhibited that the system of DES was: mole ratio between choline chloride and oxalic acid of 2:1, water content of 90% (v/v), and the optimal extraction parameters were as follows: extraction time of 31.2 min, extraction temperature of 62.1C, and the liquid-solid ratio of 32.5:1 (v/w). Under these extraction parameters, the extraction yield of MIP-D was 4.5 times higher than hot water extraction (HWE) method and had higher carbohydrate (85.27%) and sulfate contents (34.16%). Moreover, high-performance liquid chromatography (HPLC) and Fourier-transform IR (FTIR) spectrum analysis indicated that MIP-D was comprised of glucosamine, galactose, glucose, and mannose, with molar ratios of 0.39:1.88:3.82:3.91, which contained the pyranose ring skeleton. High-performance gel permeation chromatography (HPGPC) analysis revealed that MIP-D showed three fractions with molecular weights of 2.6 × 10, 7.3 × 10, and 3.7 × 10 Da, which were lower than those of polysaccharides extracted by HWE. tests proved that MIP-D possessed excellent antioxidant and inhibited α-amylase and α-glucosidase inhibitory activities. Therefore, DESs (choline chloride-oxalic acid) as a high-efficiency and non-pollution solvent alternative can be applied to the separation of bioactive polysaccharides from ().
在本研究中，一种高效无污染的提取方法——深共熔溶剂（DESs）辅助超声技术，被用于从（MIP-D）中提取多糖。结果表明，DES体系为：氯化胆碱与草酸的摩尔比为2:1，水含量为90%（v/v），最佳提取参数如下：提取时间31.2分钟，提取温度62.1℃，液固比32.5:1（v/w）。在这些提取参数下，MIP-D的提取率比热水提取（HWE）法高4.5倍，且碳水化合物含量（85.27%）和硫酸盐含量（34.16%）更高。此外，高效液相色谱（HPLC）和傅里叶变换红外（FTIR）光谱分析表明，MIP-D由氨基葡萄糖、半乳糖、葡萄糖和甘露糖组成，摩尔比为0.39:1.88:3.82:3.91，含有吡喃糖环骨架。高效凝胶渗透色谱（HPGPC）分析显示，MIP-D呈现出三个分子量分别为2.6×10、7.3×10和3.7×10 Da的组分，低于HWE法提取的多糖。测试证明，MIP-D具有优异的抗氧化性能，并具有抑制α-淀粉酶和α-葡萄糖苷酶的活性。因此，DESs（氯化胆碱-草酸）作为一种高效无污染的溶剂替代品，可用于从（）中分离生物活性多糖。
