Asero R, Arena A, Cervone M, Crivellaro M, Lodi Rizzini F, Longo R, Macchia D, Manzotti G, Minale P, Murzilli F, Polillo B R, Pravettoni V, Ridolo E, Savi E, Villalta D, Amato S, Mistrello G
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Eur Ann Allergy Clin Immunol. 2013 Sep 1;45(5):160-6.
The prevalence of IgE reactivity against genuine walnut and hazelnut allergens is poorly defined.
The IgE response to walnut and hazelnut was investigated in Italian patients with primary allergy to these nuts.
Sera from 36 patients allergic to hazelnut and/or walnut, not reactive to PR-10, profilin, and LTP, underwent immunoblot analysis with extracts of both nuts.
Most patients had a history of systemic symptoms following the ingestion of the offending food(s). Twelve patients were sensitized to both walnut and hazelnut, and 13 were sensitized to other nuts and seeds (cashew, peanut, sesame, pine nut, almond, Brazil nut, and pistachio). On walnut immunoblot, the 7 sera which scored positive showed much variability in their IgE profile. Two reacted uniquely at 10 kDa, and the others at 35 , 40, 45, 50, 67, and > 67 kDa. The profiles obtained under reducing and non-reducing conditions showed several differences. The 7 sera positive on hazelnut immunoblot under reducing conditions recognized sera at 10 kDa and at <10 kDa (n=1), 20 kDa (n=4), at about 22, 24, 30, 40, 43, 58, 60, and 90 kDa, and higher m.w. in other cases. Under non-reducing conditions IgE reactivity at 20, 28, 35, 40, 45, 60, 90, and 100 kDa, was detected. Only two sera scored positive under both conditions and showed an IgE profile that partly changed from one assay to another.
The current list of walnut and hazelnut allergens is far from being complete. Both reducing and non-reducing conditions are needed to detect IgE reactivity in individual patients.
针对真正的核桃和榛子过敏原的IgE反应性患病率定义不明确。
在对这些坚果原发性过敏的意大利患者中研究对核桃和榛子的IgE反应。
对36例对榛子和/或核桃过敏、对PR-10、肌动蛋白结合蛋白和脂质转移蛋白无反应的患者的血清，用两种坚果的提取物进行免疫印迹分析。
大多数患者在摄入致病食物后有全身症状史。12例患者对核桃和榛子均致敏，13例患者对其他坚果和种子（腰果、花生、芝麻、松子、杏仁、巴西坚果和开心果）致敏。在核桃免疫印迹中，7份呈阳性的血清在其IgE谱上显示出很大的变异性。两份血清仅在10 kDa处有反应，其他血清在35、40、45、50、67和>67 kDa处有反应。在还原和非还原条件下获得的谱图显示出一些差异。在还原条件下，7份在榛子免疫印迹上呈阳性的血清识别出10 kDa和<10 kDa（n=1）、20 kDa（n=4）、约22、24、30、40、43、58、60和90 kDa以及其他情况下更高分子量的血清。在非还原条件下，检测到20、28、35、40、45、60、90和100 kDa处的IgE反应性。只有两份血清在两种条件下均呈阳性，并且显示出从一种检测到另一种检测时IgE谱部分发生变化。
目前的核桃和榛子过敏原清单远未完整。需要还原和非还原条件来检测个体患者中的IgE反应性。
