Noumedem Jaures Ak, Tamokou Jean de Dieu, Teke Gerald Ngo, Momo Rosine Cd, Kuete Victor, Kuiate Jules Roger
Department of Biochemistry, Faculty of science, University of Dschang, P.O. Box 67, Dschang, Cameroon.
Springerplus. 2013 Oct 3;2:503. doi: 10.1186/2193-1801-2-503. eCollection 2013.
Acalypha manniana (Euphorbiaceae) is a plant popularly used in Cameroon and in several parts of Africa for the treatment of various microbial diseases like diarrhea and skin infections. The present study was designed to evaluate the phytochemical composition, antimicrobial and radical-scavenging activities of A. manniana methanol leaf extract and its fractions. The methanol extract was partitioned into hexane, ethyl acetate and residual fractions and phytochemical analysis was conducted using standard methods. The broth microdilution method was used to evaluate the antimicrobial activity against nine bacterial species and four dermatophyte species. The free radical scavenging activities of the methanol extract and its fractions were evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The results obtained showed that A. manniana contains alkaloids, tannins, anthocyanins, flavonoids, phenols and steroids. The methanol extract as well as the hexane, ethyl acetate and residual fractions exhibited both antibacterial and antidermatophytic activities that varied between the microbial species (MIC = 0.12 - 2.04 mg/mL). These tested samples also showed high radical-scavenging activities (RaS50 = 3.34 - 4.80 μg/mL) when compared with vitamin C used as reference antioxidant (RaS50 = 1.74 μg/mL). These findings provide evidence that the studied plant possesses antimicrobial and antioxidant properties and may act as potential antioxidant for biological systems susceptible to free radical-mediated reactions.
曼氏铁苋菜（大戟科）是一种在喀麦隆和非洲其他一些地区广泛用于治疗腹泻和皮肤感染等多种微生物疾病的植物。本研究旨在评估曼氏铁苋菜甲醇叶提取物及其馏分的植物化学成分、抗菌和自由基清除活性。将甲醇提取物分为己烷、乙酸乙酯和残余馏分，并采用标准方法进行植物化学分析。采用肉汤微量稀释法评估对9种细菌和4种皮肤癣菌的抗菌活性。使用2,2-二苯基-1-苦基肼（DPPH）法评估甲醇提取物及其馏分的自由基清除活性。所得结果表明，曼氏铁苋菜含有生物碱、单宁、花青素、黄酮类、酚类和甾体。甲醇提取物以及己烷、乙酸乙酯和残余馏分均表现出抗菌和抗皮肤癣菌活性，不同微生物种类之间的活性有所差异（最低抑菌浓度=0.12 - 2.04毫克/毫升）。与用作参考抗氧化剂的维生素C（半数清除率=1.74微克/毫升）相比，这些测试样品还表现出较高的自由基清除活性（半数清除率=3.34 - 4.80微克/毫升）。这些发现提供了证据，表明所研究的植物具有抗菌和抗氧化特性，对于易受自由基介导反应影响的生物系统可能具有潜在的抗氧化作用。
