Kuete Victor, Mbaveng Armelle Tsafack, Tsaffack Maurice, Beng Véronique Penlap, Etoa Francois-Xavier, Nkengfack Augustin Ephraim, Meyer J J Marion, Lall Namrita
Department of Biochemistry, Faculty of Science, University of Yaoundé I, BP 812 Yaoundé, Cameroon.
J Ethnopharmacol. 2008 Feb 12;115(3):494-501. doi: 10.1016/j.jep.2007.10.027. Epub 2007 Oct 26.
The aim of this study was to evaluate the antitumor, antioxidant and antimicrobial activities as well as the phytochemical composition and the acute toxicity of the methanolic extracts from the roots (BER), stem bark (BEB), leaves (BEL) and wood (BEW) of Bersama engleriana. The crown gall tumor and DPPH radical scavenging assays were used to detect respectively the antitumoral and oxidant activities. Agar diffusion and liquid dilution were used for antimicrobial tests and the phytochemical assays were conducted according to Harbone methods. The single-dose oral toxicity test was performed in accordance with the OPPTS 870.1100 and OECD 401 guidelines. The phytochemical tests indicated the presence of flavonoids, phenols, triterpenes and anthraquinones in all extracts. Pronounced tumor reducing activity was observed with the extracts from the roots (69.32%) and leaves (65.42%). The DPPH scavenging activity showed that the extract from the leaves was the most active with 93.71% inhibition rate at the 1000 microg/ml. The results of antimicrobial activity showed that all tested extracts were active against all tested microbial species, including Gram-positive, and negative bacteria, the two Candida species and mycobacteria. The MIC values obtained ranged from 9.76 to 156.25 microg/ml. Under the conditions of the studied toxicity, all extracts were found to be non-toxic. The overall results of this study indicates that the extracts from the roots, stem bark, leaves and wood have interesting antioxidant properties and represent a potential source of medicine for the treatment of infectious diseases and cancer.
本研究旨在评估恩格勒氏钟萼木根（BER）、茎皮（BEB）、叶（BEL）和木材（BEW）的甲醇提取物的抗肿瘤、抗氧化和抗菌活性，以及植物化学成分和急性毒性。采用冠瘿肿瘤和DPPH自由基清除试验分别检测抗肿瘤和抗氧化活性。采用琼脂扩散法和液体稀释法进行抗菌试验，并根据哈伯恩方法进行植物化学分析。单剂量口服毒性试验按照OPPTS 870.1100和经合组织401指南进行。植物化学试验表明，所有提取物中均含有黄酮类、酚类、三萜类和蒽醌类化合物。根提取物（69.32%）和叶提取物（65.42%）具有显著的肿瘤抑制活性。DPPH清除活性表明，叶提取物活性最强，在1000μg/ml时抑制率为93.71%。抗菌活性结果表明，所有受试提取物对所有受试微生物均有活性，包括革兰氏阳性菌、革兰氏阴性菌、两种念珠菌和分枝杆菌。获得的最低抑菌浓度值范围为9.76至156.25μg/ml。在所研究的毒性条件下，所有提取物均无毒。本研究的总体结果表明，根、茎皮、叶和木材的提取物具有有趣的抗氧化特性，并代表了治疗传染病和癌症的潜在药物来源。
