Yi Deliang, Wu Chengtie, Ma Bing, Ji Heng, Zheng Xuebin, Chang Jiang
1State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, China.
J Biomater Appl. 2014 May;28(9):1343-53. doi: 10.1177/0885328213508165. Epub 2013 Oct 15.
Previous studies have shown that bredigite (Ca7MgSi4O16) bioceramics possessed excellent biocompatibility, apatite-mineralization ability and mechanical properties. In this paper, the bredigite coating on Ti-6Al-4 V substrate was prepared by plasma spraying technique. The main compositions of the coating were bredigite crystal phase with small parts of amorphous phases. The bonding strength of the coating to Ti-6Al-4 V substrate reached 49.8 MPa, which was significantly higher than that of hydroxyapatite coating and other silicate-based bioceramic coatings prepared by same method. After immersed in simulated body fluid for 2 days, a distinct apatite layer was deposited on the surface of bredigite coating, indicating that the prepared bredigite coating has excellent apatite-mineralization ability. The prepared bredigite coating supported the attachment and proliferation of rabbit bone marrow stem cells. The proliferation level of bone marrow stem cells was significantly higher than that on the hydroxyapatite coating. Our further study showed that the released SiO4 (4-) and Mg(2+) ions from bredigite coating as well as the formed nano-apatite layer on the coating surface might mainly contribute to the improvement of cell proliferation. The results indicated that the bredigite coating may be applied on orthopedic implants due to its excellent bonding strength, apatite mineralization and cytocompatibility.
先前的研究表明,硅钙镁石(Ca7MgSi4O16)生物陶瓷具有优异的生物相容性、磷灰石矿化能力和机械性能。本文采用等离子喷涂技术在Ti-6Al-4V基体上制备了硅钙镁石涂层。涂层的主要成分为硅钙镁石晶相和少量非晶相。涂层与Ti-6Al-4V基体的结合强度达到49.8MPa,显著高于羟基磷灰石涂层和采用相同方法制备的其他硅酸盐基生物陶瓷涂层。将其浸泡在模拟体液中2天后,硅钙镁石涂层表面沉积了一层明显的磷灰石层,表明所制备的硅钙镁石涂层具有优异的磷灰石矿化能力。所制备的硅钙镁石涂层支持兔骨髓干细胞的附着和增殖。骨髓干细胞的增殖水平显著高于羟基磷灰石涂层上的增殖水平。我们的进一步研究表明,硅钙镁石涂层释放的SiO4(4-)和Mg(2+)离子以及涂层表面形成的纳米磷灰石层可能是促进细胞增殖的主要原因。结果表明,硅钙镁石涂层因其优异的结合强度、磷灰石矿化能力和细胞相容性,有望应用于骨科植入物。