Instituto Politécnico Nacional, Centro de Investigación en Biotecnología Aplicada, Carretera Federal Santa Inés Tecuexcomac-Tepetitla Km 1.5, C.P. 90700, Tepetitla de Lardizabal, Tlaxcala, Mexico.
World J Microbiol Biotechnol. 2014 Mar;30(3):999-1009. doi: 10.1007/s11274-013-1518-7. Epub 2013 Oct 17.
Nine native non-ligninolytic fungal strains were isolated from Maya crude oil-contaminated soil and selected based on their ability to grow and use crude oil and several polycyclic aromatic hydrocarbons (PAHs) as carbon source, for their application to PAH removal in soil. The fungi were identified by PCR amplification of intergenic transcribed sequences regions and microbiological techniques, and results showed them to be part of the genera Fusarium, Neurospora, Aspergillus, Scedosporium, Penicillium, Neosartorya and Talaromyces. A primary selection of fungi was made in minimal medium plates, considering the tolerance to different concentrations of PAHs for each strain. The radial extension rate exhibited significant differences (p < 0.05) from 200 to 1,000 mg of PAHs mixture l⁻¹. A secondary selection of Aspergillus terreus, Talaromyces spectabilis, and Fusarium sp. was achieved based on their tolerance to 2,000 mg of a mixture of Phenanathrene and Pyrene kg⁻¹ of soil in a solid-state microcosm system for 2 weeks. The percentage of PAH removal obtained by the three strains was approximately 21 % of the mixture.
从玛雅原油污染土壤中分离出 9 株非木质素分解真菌菌株，根据其利用原油和多种多环芳烃（PAHs）作为碳源生长的能力，选择这些菌株用于去除土壤中的 PAHs。通过基因间转录区 PCR 扩增和微生物学技术对真菌进行鉴定，结果表明它们属于镰孢菌属、脉孢菌属、曲霉属、枝孢属、青霉属、新萨托利菌属和拟青霉属。在最小培养基平板上对真菌进行初步筛选，考虑到每种菌株对不同浓度 PAHs 的耐受性。从 200 到 1000 mg 的 PAHs 混合物 l ⁻¹ ，径向扩展率表现出显著差异（p < 0.05）。基于它们在 2 周的固态微宇宙系统中对 2000 mg 混合苊和芘 kg ⁻¹ 的土壤的耐受性，对 Aspergillus terreus、Talaromyces spectabilis 和 Fusarium sp. 进行了二次选择。三株菌对混合物的 PAH 去除率约为 21%。
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