Ilyas A A, Quarles R H, Dalakas M C, Brady R O
Proc Natl Acad Sci U S A. 1985 Oct;82(19):6697-700. doi: 10.1073/pnas.82.19.6697.
Immunoglobulins from patients with paraproteinemic polyneuropathy were screened for reactivity with nerve and brain glycolipids by ELISA and/or a thin-layer-chromatogram-overlay technique. The myelin-associated glycoprotein (MAG) has been shown to be an antigen in many neuropathy patients with IgM gammopathy, but this study focused on seven neuropathy patients in which the IgM paraproteins had been shown not to react with this glycoprotein. Five of these seven had IgM that reacted with components in the acidic glycolipid fraction of human sciatic nerve, and three of these IgMs also reacted with components in the acidic glycolipid fraction of human brain. Little or no reactivity with glycolipids was detected for two patients with neuropathy and IgG gammopathy or for two with neuropathy and IgA gammopathy. The results suggest that neuropathy-patient IgM paraproteins not reactive with MAG often react with acidic glycolipids and thus define a subset of paraproteinemic neuropathies. Since the IgM paraproteins that react with MAG also react with acidic glycolipids of nerve, glycolipid antigens appear to be quite common among the IgM paraproteinemic neuropathies.
通过酶联免疫吸附测定（ELISA）和/或薄层层析覆盖技术，对患有副蛋白血症性多发性神经病患者的免疫球蛋白与神经和脑糖脂的反应性进行了筛查。髓鞘相关糖蛋白（MAG）已被证明是许多患有IgM丙种球蛋白病的神经病患者的一种抗原，但本研究聚焦于7名神经病患者，其IgM副蛋白已被证明与这种糖蛋白无反应。这7名患者中有5名的IgM与人类坐骨神经酸性糖脂部分的成分发生反应，其中3名患者的IgM还与人类脑酸性糖脂部分的成分发生反应。对于2名患有神经病和IgG丙种球蛋白病的患者或2名患有神经病和IgA丙种球蛋白病的患者，未检测到与糖脂的反应或反应较弱。结果表明，与MAG无反应的神经病患者IgM副蛋白通常与酸性糖脂发生反应，从而确定了副蛋白血症性神经病的一个子集。由于与MAG发生反应的IgM副蛋白也与神经酸性糖脂发生反应，糖脂抗原在IgM副蛋白血症性神经病中似乎相当常见。
