Hacker J, Hughes C, Hof H, Goebel W
Infect Immun. 1983 Oct;42(1):57-63. doi: 10.1128/iai.42.1.57-63.1983.
After intraperitoneal injection of mice with Escherichia coli strains isolated from patients with urinary tract infections, the mortality due to hemolytic (Hly+) and nonhemolytic (Hly-) isolates was 77 and 40%, respectively. Deletion of the chromosomal hemolysin (hly) determinant in an E. coli O6:K15:H31 urinary tract infection strain led to a significant reduction in toxicity for mice, and its reintroduction on a recombinant plasmid partially restored the original toxicity. Although introduction of the cloned plasmid pHly152-encoded hly determinant into the Hly- E. coli O6 mutant strain increased toxicity by only a marginal degree, transformation with the cloned chromosomal hly determinants from two E. coli strains of serotypes O18ac:K5:H- and O75:K95:H? resulted in markedly greater toxicity, even exceeding that of the original Hly+ E. coli O6 wild-type strain.
给小鼠腹腔注射从尿路感染患者分离出的大肠杆菌菌株后，溶血（Hly+）和非溶血（Hly-）分离株导致的死亡率分别为77%和40%。在一株大肠杆菌O6:K15:H31尿路感染菌株中删除染色体溶血素（hly）决定簇，导致对小鼠的毒性显著降低，而将其重新导入重组质粒可部分恢复原始毒性。虽然将克隆质粒pHly152编码的hly决定簇导入Hly-大肠杆菌O6突变株仅使毒性略有增加，但用来自血清型O18ac:K5:H-和O75:K95:H?的两株大肠杆菌菌株的克隆染色体hly决定簇进行转化，导致毒性明显更大，甚至超过了原始的Hly+大肠杆菌O6野生型菌株。
