Chung Yuen-Li, El Akabawy Gehan, So Po-Wah, Solanky Bhavana S, Leach Martin O, Modo Michel
aCancer Research UK and EPSRC Cancer Imaging Centre, Institute of Cancer Research and Royal Marsden Hospital, Surrey Departments of bNeuroscience cNeuroimaging, Institute of Psychiatry, Kings College London, London, UK dDepartment of Anatomy and Embryology, Faculty of Medicine, University of Menoufia, Menoufia, Egypt eDepartment of Radiology, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
Neuroreport. 2013 Dec 18;24(18):1035-40. doi: 10.1097/WNR.0000000000000056.
Neural stem cells (NSCs) have been found to play an increasing clinical role in stroke. However, at present, it is not yet possible to noninvasively monitor their differentiation once implanted into the brain.
Here, we describe the use of high-resolution H-magnetic resonance spectroscopy (MRS) to define a metabolite profile of undifferentiated human striatal NSCs from the STROC05 cell line and their differentiation after 3-weeks of treatment with purmorphamine.
The undifferentiated conditions were characterized by 95% of cells expressing nestin and ~77% being Ki67(+)ve, indicating that these were still proliferating. Phosphophocholine+glycerophosphocholine (PC+GPC) as well as myo-Inositol (mI) were increased in these cells. PC+GPC and mI were markedly reduced upon differentiation, potentially serving as markers of the NSC state. Upon differentiation (45% neurons, ~30% astrocytes, ~13% oligodendrocytes), the concentration of many metabolites decreased in absolute value. The decreasing trend of the N-acetyl-aspartate level was observed in differentiated cells when compared with NSCs. An increase in plasmalogen (enriched in myelin sheets) could potentially serve as a marker of oligodendrocytes.
These metabolite characteristics of undifferentiated and differentiated NSCs provide a basis for exploration of their possible use as markers of differentiation after cell transplantation.
神经干细胞（NSCs）在中风治疗中的临床作用日益显著。然而，目前尚无法在植入大脑后对其分化情况进行无创监测。
在此，我们描述了利用高分辨率氢磁共振波谱（MRS）来确定来自STROC05细胞系的未分化人纹状体神经干细胞的代谢物谱及其在用嘌呤吗啡治疗3周后的分化情况。
未分化状态的特征为约95%的细胞表达巢蛋白，约77%为Ki67阳性，表明这些细胞仍在增殖。这些细胞中的磷酸胆碱+甘油磷酸胆碱（PC+GPC）以及肌醇（mI）有所增加。分化后PC+GPC和mI显著降低，可能作为神经干细胞状态的标志物。分化后（约45%为神经元，约30%为星形胶质细胞，约13%为少突胶质细胞），许多代谢物的浓度绝对值下降。与神经干细胞相比，分化细胞中N-乙酰天门冬氨酸水平呈下降趋势。缩醛磷脂（富含髓鞘）的增加可能作为少突胶质细胞的标志物。
未分化和分化神经干细胞的这些代谢物特征为探索其作为细胞移植后分化标志物的可能用途提供了基础。
