Orozco-Barocio Arturo, Paniagua-Domínguez Brenda Lizbeth, Benítez-Saldaña Pedro Alberto, Flores-Torales Edgardo, Velázquez-Magaña Salvador, Nava Hilda Julieta Arreola
Laboratory of Immunobiology, Department of Cellular and Molecular Biology. University Center of Biological and Agricultural Sciences. University of Guadalajara. Km 15.5 carretera Guadalajara - Nogales, Las Agujas, Nextipac, Zapopan, Jalisco, México. CP: 44100.
Afr J Tradit Complement Altern Med. 2013 Apr 12;10(3):397-404. eCollection 2013.
Lophocereus schottii is a Mexican cactus known as garambullo whose bark is used for the treatment of cancer, diabetes, ulcers, sores, stomach disorders and tuberculosis. The aim of this study was to evaluate the cytotoxic effect of the ethanolic extract of bark of L. Schottii. To assess these effects we established a flow of experiments in a model of BALB/c mice murine lymphoma. We value first survival of mice inoculated with 2 × 10(4) L5178Y murine lymphoma cells, orally treated with 10 mg/Kg of the extract for 10 consecutive days; the second assessment was to determine the influence of the immune system, we carry out studies of lymphoproliferation in mice with the same conditions of the previous study, only that the treatment was for 22 days before the completion cell cultures; the third study was to establish the cytotoxic effect of extract of L. schottii using different concentrations, by murine lymphoma cell cultures and splenocytes from healthy mice and finally we assessed the effect in vivo of extract of L. Schottii in a model of solid murine lymphoma inoculating 1 × 10(7) lymphoma cells in the gastrocnemius muscle observing the development of the tumor. We observed that oral treatment of 10 mg/kg of extract of L. schottii increased survival rate in treated mice; additionally, an intratumoral injection of 50 and 100 mg/kg in a solid murine lymphoma located in the gastrocnemius muscle, allowed a significantly slower tumor evolution. In vitro studies determined that extract inhibited 63% of lymphoma cell growth. With these evidences it is feasible to scientifically validate that ethanolic extract of L. schottii had an effect on L5178Y murine cells lymphoma and could have the same effect in human tumors.
肖特氏鸡冠柱是一种名为加拉姆布洛的墨西哥仙人掌，其树皮可用于治疗癌症、糖尿病、溃疡、疮、胃部疾病和肺结核。本研究的目的是评估肖特氏鸡冠柱树皮乙醇提取物的细胞毒性作用。为了评估这些作用，我们在BALB/c小鼠鼠淋巴瘤模型中建立了一系列实验流程。我们首先评估接种2×10⁴个L5178Y鼠淋巴瘤细胞的小鼠的存活率，这些小鼠连续10天口服10mg/Kg的提取物；第二项评估是确定免疫系统的影响，我们在与先前研究相同条件的小鼠中进行淋巴细胞增殖研究，只是治疗在细胞培养完成前持续22天；第三项研究是通过小鼠淋巴瘤细胞培养和健康小鼠的脾细胞，使用不同浓度来确定肖特氏鸡冠柱提取物的细胞毒性作用，最后我们在实体鼠淋巴瘤模型中评估肖特氏鸡冠柱提取物的体内作用，在腓肠肌接种1×10⁷个淋巴瘤细胞，观察肿瘤的发展。我们观察到口服10mg/kg的肖特氏鸡冠柱提取物可提高治疗小鼠的存活率；此外，在位于腓肠肌的实体鼠淋巴瘤中瘤内注射50和100mg/kg，可使肿瘤进展明显减缓。体外研究确定该提取物抑制了63%的淋巴瘤细胞生长。有了这些证据，科学验证肖特氏鸡冠柱乙醇提取物对L5178Y鼠细胞淋巴瘤有作用且可能对人类肿瘤有相同作用是可行的。
