Navarro-Salcedo Martha Hilda, Delgado-Saucedo Jorge Ivan, Siordia-Sánchez Victor Hugo, González-Ortiz Luis J, Castillo-Herrera Gustavo Adolfo, Puebla-Pérez Ana M
1 Pharmacobiology Department, University Center of Exact Science and Engineering, University of Guadalajara , Guadalajara, Jalisco, México.
2 Chemistry Department, University Center of Exact Science and Engineering, University of Guadalajara , Guadalajara, Jalisco, México.
J Med Food. 2017 Nov;20(11):1076-1082. doi: 10.1089/jmf.2017.0044. Epub 2017 Jul 24.
We investigated the cytotoxic and antitumor effects of nine leaf extracts from Artemisia dracunculus (Tarragon). Five extracts were obtained using different organic solvents and four by supercritical CO. The cytotoxic effects were expressed as IC in 100, 80, 80, 100, and 80 μg/mL by respective solvents: hexane, ethyl acetate, acetone, ethanol, and acetonitrile in L5178Y lymphoma cells. For supercritical CO extract A, IC was 100 μg/mL; for extracts C and D, IC was 150 μg/mL. The antitumor activity was assessed through a tumor growth inhibition test that measured ascites fluid volume and tumor cell counts of BALB/c mice (2 × 10 cells L5178Y i.p.). Twenty-four hours after inoculation, mice were treated with 100 mg/kg of acetonitrile extract or extract SF-A daily for 15 days in independent groups of five mice, using two administration routes. We observed tumor evolution with and without treatment. Without treatment, tumor evolution was 17,969 × 10 ± 5485 L5178Y cells in 2.6 mL ascites volume, whereas the orally treated acetonitrile extract group showed 0.1 × 10 ± 0.07 L5178Y cells (P < .05). The oral SF-A group showed 12.9 × 10 ± 243 L5178Y cells, and intraperitoneal (i.p.)-treated SF-A group showed 0.1 × 10 ± 0.05 L5178Y cells (P < .05) without any ascites volume development. The acetonitrile extract contains abundant polyphenols and possibly a flavone with antioxidant activity. The SF-A contains abundant alkamides. Both extracts are complexes and the identity of the compounds responsible for observed antitumor activity remains unknown.
我们研究了龙蒿（狭叶青蒿）九种叶提取物的细胞毒性和抗肿瘤作用。其中五种提取物是使用不同有机溶剂获得的，另外四种是通过超临界CO₂提取的。细胞毒性作用以L5178Y淋巴瘤细胞中分别用己烷、乙酸乙酯、丙酮、乙醇和乙腈作为溶剂时的IC₅₀表示，浓度分别为100、80、80、100和80μg/mL。对于超临界CO₂提取物A，IC₅₀为100μg/mL；对于提取物C和D，IC₅₀为150μg/mL。通过肿瘤生长抑制试验评估抗肿瘤活性，该试验测量BALB/c小鼠（2×10⁶个L5178Y细胞腹腔注射）的腹水体积和肿瘤细胞计数。接种后24小时，将小鼠分为独立的五组，每组五只，分别通过两种给药途径每天用100mg/kg的乙腈提取物或提取物SF-A处理15天。我们观察了有无治疗情况下的肿瘤进展。未治疗时，肿瘤进展为2.6mL腹水中有17,969×10⁶±5485个L5178Y细胞，而口服乙腈提取物治疗组显示有0.1×10⁶±0.07个L5178Y细胞（P＜0.05）。口服SF-A组显示有12.9×10⁶±243个L5178Y细胞，腹腔注射（i.p.）治疗的SF-A组显示有0.1×10⁶±0.05个L5178Y细胞（P＜0.05），且没有腹水形成。乙腈提取物含有丰富的多酚类物质，可能还有一种具有抗氧化活性的黄酮类化合物。SF-A含有丰富的链状酰胺。两种提取物都是复合物，负责观察到的抗肿瘤活性的化合物身份仍然未知。
