Orozco-Barocio Arturo, Robles-Rodríguez Blanca Susana, Camacho-Corona María Del Rayo, Méndez-López Luis Fernando, Godínez-Rubí Marisol, Peregrina-Sandoval Jorge, Rivera Gildardo, Rojas Mayorquín Argelia E, Ortuno-Sahagun Daniel
Laboratorio de Inmunobiología, Departamento de Biología Celular y Molecular, Universidad de Guadalajara, Centro Universitario de Ciencias Biológicas y Agropecuarias, Zapopan, Mexico.
Facultad de Ciencias Químicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Mexico.
Front Pharmacol. 2022 Apr 4;13:820381. doi: 10.3389/fphar.2022.820381. eCollection 2022.
Cancer is an increasingly common disease and is considered one of the main causes of death in the world. () is a cactus used in Mexico in traditional medicine for cancer treatment. This study aimed to determine the effect of the ethanolic extract and the polar and nonpolar fractions of in murine L5178Y lymphoma cells , analyzing their effect on the proliferative activity of splenocytes, and establishing the effective concentration 50 (EC) of the polar fraction. In addition, the secondary metabolites present in the extracts were determined by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). The study establishes that the three extracts of have a cytotoxic effect on L5178Y cells and on the splenocytes stimulated with ConA. Additionally, the polar fraction has a significantly greater effect being three times more effective than cyclophosphamide on inhibiting the viability of L5178Y cells. Secondary metabolites present are mainly flavonoids and alkaloids, but there are also some terpenoids and sterols. Ultimately, polar fraction can be considered an anticancer substance, since its EC of 15 μg/mL is within the parameters established by the National Cancer Institute.
癌症是一种日益常见的疾病，被认为是世界上主要的死亡原因之一。（）是一种在墨西哥传统医学中用于治疗癌症的仙人掌。本研究旨在确定该仙人掌乙醇提取物及其极性和非极性组分对小鼠L5178Y淋巴瘤细胞的影响，分析它们对脾细胞增殖活性的作用，并确定极性组分的半数有效浓度（EC50）。此外，通过超高效液相色谱-质谱联用仪（UPLC-MS）测定提取物中存在的次生代谢产物。该研究表明，该仙人掌的三种提取物对L5178Y细胞以及用刀豆蛋白A刺激的脾细胞具有细胞毒性作用。此外，极性组分的作用明显更大，在抑制L5178Y细胞活力方面比环磷酰胺有效三倍。存在的次生代谢产物主要是黄酮类化合物和生物碱，但也有一些萜类化合物和甾醇。最终，极性组分可被视为一种抗癌物质，因为其15μg/mL的EC50在国家癌症研究所确定的参数范围内。
