• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

参与肺炎链球菌隔膜细胞壁组装的膜蛋白复合物的重组。

Reconstitution of membrane protein complexes involved in pneumococcal septal cell wall assembly.

作者信息

Noirclerc-Savoye Marjolaine, Lantez Violaine, Signor Luca, Philippe Jules, Vernet Thierry, Zapun André

机构信息

Institut de Biologie Structurale, Université Grenoble Alpes, Grenoble, France ; Institut de Biologie Structurale, Direction des Sciences du Vivant, Commissariat à l'energie atomique et aux Energies Alternatives, Grenoble, France ; Institut de Biologie Structurale, Centre National de la Recherche Scientifique, Grenoble, France.

出版信息

PLoS One. 2013 Sep 23;8(9):e75522. doi: 10.1371/journal.pone.0075522. eCollection 2013.

DOI:10.1371/journal.pone.0075522
PMID:24147156
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3798694/
Abstract

The synthesis of peptidoglycan, the major component of the bacterial cell wall, is essential to cell survival, yet its mechanism remains poorly understood. In the present work, we have isolated several membrane protein complexes consisting of the late division proteins of Streptococcus pneumoniae: DivIB, DivIC, FtsL, PBP2x and FtsW, or subsets thereof. We have co-expressed membrane proteins from S. pneumoniae in Escherichia coli. By combining two successive affinity chromatography steps, we obtained membrane protein complexes with a very good purity. These complexes are functional, as indicated by the retained activity of PBP2x to bind a fluorescent derivative of penicillin and to hydrolyze the substrate analogue S2d. Moreover, we have evidenced the stabilizing role of protein-protein interactions within each complex. This work paves the way for a complete reconstitution of peptidoglycan synthesis in vitro, which will be critical to the elucidation of its intricate regulation mechanisms.

摘要

肽聚糖是细菌细胞壁的主要成分，其合成对细胞存活至关重要，但其机制仍知之甚少。在本研究中，我们分离出了几种由肺炎链球菌的后期分裂蛋白组成的膜蛋白复合物：DivIB、DivIC、FtsL、PBP2x和FtsW，或其亚组。我们已在大肠杆菌中共表达了肺炎链球菌的膜蛋白。通过连续两步亲和层析，我们获得了纯度非常高的膜蛋白复合物。这些复合物具有功能，PBP2x结合青霉素荧光衍生物并水解底物类似物S2d的活性保留就表明了这一点。此外，我们还证明了每个复合物中蛋白质 - 蛋白质相互作用的稳定作用。这项工作为体外完全重建肽聚糖合成铺平了道路，这对于阐明其复杂的调控机制至关重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c58f/3798694/633ea7833fed/pone.0075522.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c58f/3798694/04b11d0c0cf9/pone.0075522.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c58f/3798694/fd3d19883e03/pone.0075522.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c58f/3798694/01a3e5952958/pone.0075522.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c58f/3798694/633ea7833fed/pone.0075522.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c58f/3798694/04b11d0c0cf9/pone.0075522.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c58f/3798694/fd3d19883e03/pone.0075522.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c58f/3798694/01a3e5952958/pone.0075522.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c58f/3798694/633ea7833fed/pone.0075522.g004.jpg

相似文献

1
Reconstitution of membrane protein complexes involved in pneumococcal septal cell wall assembly.参与肺炎链球菌隔膜细胞壁组装的膜蛋白复合物的重组。
PLoS One. 2013 Sep 23;8(9):e75522. doi: 10.1371/journal.pone.0075522. eCollection 2013.
2
In vitro reconstitution of a trimeric complex of DivIB, DivIC and FtsL, and their transient co-localization at the division site in Streptococcus pneumoniae.肺炎链球菌中DivIB、DivIC和FtsL三聚体复合物的体外重组及其在分裂位点的瞬时共定位。
Mol Microbiol. 2005 Jan;55(2):413-24. doi: 10.1111/j.1365-2958.2004.04408.x.
3
Class A PBPs have a distinct and unique role in the construction of the pneumococcal cell wall.A 类青霉素结合蛋白在肺炎链球菌细胞壁的构建中具有独特而重要的作用。
Proc Natl Acad Sci U S A. 2020 Mar 17;117(11):6129-6138. doi: 10.1073/pnas.1917820117. Epub 2020 Mar 2.
4
Roles of pneumococcal DivIB in cell division.肺炎球菌DivIB在细胞分裂中的作用。
J Bacteriol. 2008 Jul;190(13):4501-11. doi: 10.1128/JB.00376-08. Epub 2008 Apr 25.
5
Suppression and synthetic-lethal genetic relationships of ΔgpsB mutations indicate that GpsB mediates protein phosphorylation and penicillin-binding protein interactions in Streptococcus pneumoniae D39.ΔgpsB突变的抑制和合成致死遗传关系表明，GpsB在肺炎链球菌D39中介导蛋白质磷酸化和青霉素结合蛋白相互作用。
Mol Microbiol. 2017 Mar;103(6):931-957. doi: 10.1111/mmi.13613. Epub 2017 Feb 7.
6
Movement dynamics of divisome proteins and PBP2x:FtsW in cells of .divisome 蛋白和 PBP2x:FtsW 在 . 细胞中的运动动力学
Proc Natl Acad Sci U S A. 2019 Feb 19;116(8):3211-3220. doi: 10.1073/pnas.1816018116. Epub 2019 Feb 4.
7
The cell cycle regulator GpsB functions as cytosolic adaptor for multiple cell wall enzymes.细胞周期调控因子 GpsB 作为多种细胞壁酶的细胞质衔接子发挥作用。
Nat Commun. 2019 Jan 16;10(1):261. doi: 10.1038/s41467-018-08056-2.
8
The Staphylococcus aureus cell division protein, DivIC, interacts with the cell wall and controls its biosynthesis.金黄色葡萄球菌细胞分裂蛋白DivIC与细胞壁相互作用并控制其生物合成。
Commun Biol. 2022 Nov 11;5(1):1228. doi: 10.1038/s42003-022-04161-7.
9
DivIVA controls the dynamics of septum splitting and cell elongation in .DivIVA 控制隔膜分裂和细胞伸长的动力学。
mBio. 2024 Oct 16;15(10):e0131124. doi: 10.1128/mbio.01311-24. Epub 2024 Sep 17.
10
Central domain of DivIB caps the C-terminal regions of the FtsL/DivIC coiled-coil rod.DivIB的中央结构域覆盖FtsL/DivIC卷曲螺旋杆的C末端区域。
J Biol Chem. 2009 Oct 2;284(40):27687-700. doi: 10.1074/jbc.M109.019471. Epub 2009 Jul 27.

引用本文的文献

1
Transient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complex.感受态肺炎球菌细胞中细胞分裂的短暂抑制是由隔膜肽聚糖复合物的减速引起的。
Nat Commun. 2025 Jul 1;16(1):5666. doi: 10.1038/s41467-025-60600-z.
2
SNARE-Mediated Membrane Fusion Probed Using a Synthetic Organelle in the Living Bacterium.利用活细菌中的合成细胞器探究SNARE介导的膜融合
Methods Mol Biol. 2025;2887:263-280. doi: 10.1007/978-1-0716-4314-3_19.
3
A conserved subcomplex within the bacterial cytokinetic ring activates cell wall synthesis by the FtsW-FtsI synthase.

本文引用的文献

1
Effects of low PBP2b levels on cell morphology and peptidoglycan composition in Streptococcus pneumoniae R6.低水平 PBP2b 对肺炎链球菌 R6 细胞形态和肽聚糖组成的影响。
J Bacteriol. 2013 Oct;195(19):4342-54. doi: 10.1128/JB.00184-13. Epub 2013 Jul 19.
2
From models to pathogens: how much have we learned about Streptococcus pneumoniae cell division?从模型到病原体：我们对肺炎链球菌细胞分裂了解多少？
Environ Microbiol. 2013 Dec;15(12):3133-57. doi: 10.1111/1462-2920.12189. Epub 2013 Jul 15.
3
Structural organization of FtsB, a transmembrane protein of the bacterial divisome.
细菌细胞分裂环内的一个保守亚基复合物通过 FtsW-FtsI 合成酶激活细胞壁合成。
Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23879-23885. doi: 10.1073/pnas.2004598117. Epub 2020 Sep 9.
4
Regulation of the Peptidoglycan Polymerase Activity of PBP1b by Antagonist Actions of the Core Divisome Proteins FtsBLQ and FtsN.通过核心分裂体蛋白 FtsBLQ 和 FtsN 的拮抗作用调节 PBP1b 的肽聚糖聚合酶活性。
mBio. 2019 Jan 8;10(1):e01912-18. doi: 10.1128/mBio.01912-18.
5
Interplay between Penicillin-binding proteins and SEDS proteins promotes bacterial cell wall synthesis.青霉素结合蛋白与 SEDS 蛋白之间的相互作用促进了细菌细胞壁的合成。
Sci Rep. 2017 Feb 24;7:43306. doi: 10.1038/srep43306.
6
Substitutions in PBP2b from β-Lactam-resistant Have Different Effects on Enzymatic Activity and Drug Reactivity.耐β-内酰胺菌株中PBP2b的替代对酶活性和药物反应性有不同影响。
J Biol Chem. 2017 Feb 17;292(7):2854-2865. doi: 10.1074/jbc.M116.764696. Epub 2017 Jan 6.
7
Roles for both FtsA and the FtsBLQ subcomplex in FtsN-stimulated cell constriction in Escherichia coli.FtsA和FtsBLQ亚复合物在大肠杆菌中FtsN刺激的细胞缢缩中的作用。
Mol Microbiol. 2015 Mar;95(6):945-70. doi: 10.1111/mmi.12906. Epub 2015 Jan 24.
8
The elongation of ovococci.卵圆球菌的伸长。
Microb Drug Resist. 2014 Jun;20(3):215-21. doi: 10.1089/mdr.2014.0032. Epub 2014 Apr 28.
细菌分裂体中跨膜蛋白 FtsB 的结构组织。
Biochemistry. 2013 Apr 16;52(15):2574-85. doi: 10.1021/bi400222r. Epub 2013 Apr 4.
4
Forming cross-linked peptidoglycan from synthetic gram-negative Lipid II.从合成的革兰氏阴性脂类 II 形成交联的肽聚糖。
J Am Chem Soc. 2013 Mar 27;135(12):4632-5. doi: 10.1021/ja312510m. Epub 2013 Mar 13.
5
Towards a bottom-up reconstitution of bacterial cell division.朝着细菌细胞分裂的自下而上重建迈进。
Trends Cell Biol. 2012 Dec;22(12):634-43. doi: 10.1016/j.tcb.2012.09.003. Epub 2012 Oct 12.
6
PatA and PatB form a functional heterodimeric ABC multidrug efflux transporter responsible for the resistance of Streptococcus pneumoniae to fluoroquinolones.PatA 和 PatB 形成一种功能性异二聚体 ABC 多药外排转运蛋白，负责肺炎链球菌对氟喹诺酮类药物的耐药性。
Biochemistry. 2012 Oct 2;51(39):7755-65. doi: 10.1021/bi300762p. Epub 2012 Sep 21.
7
Cooperativity of peptidoglycan synthases active in bacterial cell elongation.参与细菌细胞伸长的肽聚糖合成酶的协同作用。
Mol Microbiol. 2012 Jul;85(1):179-94. doi: 10.1111/j.1365-2958.2012.08103.x. Epub 2012 Jun 5.
8
The membrane anchor of penicillin-binding protein PBP2a from Streptococcus pneumoniae influences peptidoglycan chain length.肺炎链球菌青霉素结合蛋白 PBP2a 的膜锚定影响肽聚糖链长度。
FEBS J. 2012 Jun;279(11):2071-81. doi: 10.1111/j.1742-4658.2012.08592.x. Epub 2012 May 8.
9
HPLC-SEC characterization of membrane protein-detergent complexes.膜蛋白 - 去污剂复合物的高效液相色谱 - 尺寸排阻色谱表征
Curr Protoc Protein Sci. 2012 Apr;Chapter 29:29.5.1-29.5.12. doi: 10.1002/0471140864.ps2905s68.
10
Peptidoglycan assembly machines: the biochemical evidence.肽聚糖装配机器：生化证据。
Microb Drug Resist. 2012 Jun;18(3):256-60. doi: 10.1089/mdr.2011.0236. Epub 2012 Mar 20.