College of Food and Bioengineering, South China University of Technology, Guangzhou, 510640, China.
Food Funct. 2013 Dec;4(12):1811-8. doi: 10.1039/c3fo60342c. Epub 2013 Oct 22.
Penthorum chinense Pursh is rich in flavonoids, which have strong antioxidant and anticomplement activities. In order to optimize their extraction conditions, various extraction parameters were chosen to identify their effects on flavonoids extraction. Single factor and Box-Behnken experimental designs consisting of 24 experimental runs and five replicates at zero point were applied to obtain the optimal extraction yield. The optimization conditions for flavonoids extraction were determined as follows: ethanol concentration 60.89%, extraction time 68.15 min, temperature 52.89 °C and liquid/solid ratio 19.70 : 1. The corresponding flavonoids content was 7.19%. The regression equation was found to fit well with the actual situation. Furthermore, the antioxidant activity (the free radical scavenging ability and ferric reducing/antioxidant power) and anticomplement ability of the flavonoids from P. chinense were determined. Results showed that the flavonoids of P. chinense displayed significant antioxidant and anticomplement activities. Its antioxidant activity can compete with ascorbic acid (Vc), whereas its anticomplement activity (IC50 = 111.6 μg ml(-1)) surpassed the effect of heparin (IC50 = 399.7 μg ml(-1)) which was used as the positive control, suggesting that P. chinense flavonoids and their related products could potentially be used as a promising natural agent in the treatment of humoral effector inflammation.
对叶过路黄含有丰富的黄酮类化合物，具有较强的抗氧化和抗补体活性。为了优化其提取条件，选择了各种提取参数来确定它们对黄酮类化合物提取的影响。采用单因素和 Box-Behnken 实验设计，共进行了 24 次实验和 5 次零点重复，以获得最佳提取产率。黄酮类化合物提取的优化条件确定为：乙醇浓度 60.89%，提取时间 68.15 min，温度 52.89 °C，液固比 19.70:1。相应的黄酮类化合物含量为 7.19%。回归方程与实际情况拟合良好。此外，还测定了对叶过路黄黄酮类化合物的抗氧化活性（自由基清除能力和铁还原/抗氧化能力）和抗补体活性。结果表明，对叶过路黄的黄酮类化合物具有显著的抗氧化和抗补体活性。其抗氧化活性可与抗坏血酸（Vc）相媲美，而其抗补体活性（IC50 = 111.6 μg ml(-1)）超过了肝素（IC50 = 399.7 μg ml(-1)）的效果，肝素被用作阳性对照，这表明对叶过路黄黄酮类化合物及其相关产品可能有望成为治疗体液效应炎症的一种有前途的天然药物。
