Tarn Mark D, Lopez-Martinez Maria J, Pamme Nicole
Department of Chemistry, The University of Hull, Cottingham Road, Hull, HU6 7RX, UK.
Anal Bioanal Chem. 2014 Jan;406(1):139-61. doi: 10.1007/s00216-013-7363-6. Epub 2013 Oct 23.
The processing of particles, cells, and droplets for reactions, analyses, labeling, and coating is an important aspect of many microfluidic workflows. However, performing multi-step processes is typically a laborious and time-consuming endeavor. By exploiting the laminar nature of flow within microchannels, such procedures can benefit in terms of both speed and simplicity. This can be achieved either by manipulating the flow streams around the objects of interest, particularly for the localized perfusion of cells, or by manipulating the objects themselves within the streams via a range of forces. Here, we review the variety of methods that have been employed for performing such "multilaminar flow" procedures on particles, cells, and droplets.
对颗粒、细胞和液滴进行反应、分析、标记和包被处理是许多微流控工作流程的一个重要方面。然而，执行多步骤过程通常是一项费力且耗时的工作。通过利用微通道内流动的层流特性，此类程序在速度和简便性方面都能受益。这既可以通过操纵感兴趣对象周围的流动流来实现，特别是对于细胞的局部灌注，也可以通过一系列力在流中操纵对象本身来实现。在此，我们综述了已用于对颗粒、细胞和液滴执行此类“多层流”程序的各种方法。
