Department of NanoEngineering, University of California, MC-0448, SME Building 245B, 9500 Gilman Drive, La Jolla, San Diego, CA, 92093, USA.
Biomed Microdevices. 2014 Feb;16(1):127-32. doi: 10.1007/s10544-013-9812-6.
To understand the physical behavior and migration of cancer cells, a 3D in vitro micro-chip in hydrogel was created using 3D projection printing. The micro-chip has a honeycomb branched structure, aiming to mimic 3D vascular morphology to test, monitor, and analyze differences in the behavior of cancer cells (i.e. HeLa) vs. non-cancerous cell lines (i.e. 10 T1/2). The 3D Projection Printing system can fabricate complex structures in seconds from user-created designs. The fabricated microstructures have three different channel widths of 25, 45, and 120 microns wide to reflect a range of blood vessel diameters. HeLa and 10 T1/2 cells seeded within the micro-chip were then analyzed for morphology and cell migration speed. 10 T1/2 cells exhibited greater changes in morphology due to channel size width than HeLa cells; however, channel width had a limited effect on 10 T1/2 cell migration while HeLa cancer cell migration increased as channel width decreased. This physiologically relevant 3D cancer tissue model has the potential to be a powerful tool for future drug discoveries and cancer migration studies.
为了了解癌细胞的物理行为和迁移，我们使用三维投影印刷技术在水凝胶中创建了一个 3D 体外微芯片。该微芯片具有蜂窝状分支结构，旨在模拟 3D 血管形态，以测试、监测和分析癌细胞（如 HeLa）与非癌细胞系（如 10 T1/2）之间行为的差异。3D 投影打印系统可以根据用户创建的设计在几秒钟内制造出复杂的结构。所制造的微结构具有三个不同的通道宽度，分别为 25、45 和 120 微米，以反映一系列血管直径。然后，对微芯片内接种的 HeLa 和 10 T1/2 细胞进行形态和细胞迁移速度分析。由于通道尺寸宽度的原因，10 T1/2 细胞的形态变化比 HeLa 细胞更大；然而，通道宽度对 10 T1/2 细胞迁移的影响有限，而 HeLa 癌细胞的迁移则随着通道宽度的减小而增加。这种具有生理相关性的 3D 癌症组织模型有可能成为未来药物发现和癌症迁移研究的有力工具。
