• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

普遍应激蛋白在探测复杂对苯二甲酸宏基因组中有效降解物的动态变化中的应用。

Application of universal stress proteins in probing the dynamics of potent degraders in complex terephthalate metagenome.

机构信息

Center for Bioinformatics & Computational Biology, Department of Biology, Jackson State University, Jackson, MS 39217, USA.

出版信息

Biomed Res Int. 2013;2013:196409. doi: 10.1155/2013/196409. Epub 2013 Sep 10.

DOI:10.1155/2013/196409
PMID:24151583
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3782759/
Abstract

The culture-independent strategies to study microbial diversity and function have led to a revolution in environmental genomics, enabling fundamental questions about the distribution of microbes and their influence on bioremediation to be addressed. In this research we used the expression of universal stress proteins as a probe to determine the changes in degrading microbial population from a highly toxic terephthalate wastewater to a less toxic activated sludge bioreactor. The impact of relative toxicities was significantly elaborated at the levels of genus and species. The results indicated that 23 similar prokaryotic phyla were represented in both metagenomes irrespective of their relative abundance. Furthermore, the following bacteria taxa Micromonosporaceae, Streptomyces, Cyanothece sp. PCC 7822, Alicyclobacillus acidocaldarius, Bacillus halodurans, Leuconostoc mesenteroides, Lactococcus garvieae, Brucellaceae, Ralstonia solanacearum, Verminephrobacter eiseniae, Azoarcus, Acidithiobacillus ferrooxidans, Francisella tularensis, Methanothermus fervidus, and Methanocorpusculum labreanum were represented only in the activated sludge bioreactor. These highly dynamic microbes could serve as taxonomic biomarkers for toxic thresholds related to terephthalate and its derivatives. This paper, highlights the application of universal stress proteins in metagenomics analysis. Dynamics of microbial consortium of this nature can have future in biotechnological applications in bioremediation of toxic chemicals and radionuclides.

摘要

非培养策略研究微生物多样性和功能已经引发了环境基因组学的革命，使人们能够解决有关微生物分布及其对生物修复影响的基本问题。在这项研究中，我们使用普遍应激蛋白的表达作为探针，以确定从高毒性对苯二甲酸废水中到毒性较低的活性污泥生物反应器中降解微生物种群的变化。相对毒性的影响在属和种的水平上得到了详细阐述。结果表明，23 个相似的原核门在两个宏基因组中都有代表，而不管它们的相对丰度如何。此外，以下细菌分类群 Micromonosporaceae、Streptomyces、Cyanothece sp. PCC 7822、Alicyclobacillus acidocaldarius、Bacillus halodurans、Leuconostoc mesenteroides、Lactococcus garvieae、Brucellaceae、Ralstonia solanacearum、Verminephrobacter eiseniae、Azoarcus、Acidithiobacillus ferrooxidans、Francisella tularensis、Methanothermus fervidus 和 Methanocorpusculum labreanum 仅在活性污泥生物反应器中被代表。这些高度动态的微生物可以作为与对苯二甲酸及其衍生物相关的毒性阈值的分类生物标志物。本文强调了普遍应激蛋白在宏基因组分析中的应用。这种性质的微生物联合体的动态变化可能在生物修复有毒化学品和放射性核素的生物技术应用中具有未来。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a741/3782759/e44f3665c08d/BMRI2013-196409.011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a741/3782759/cc1396520d5a/BMRI2013-196409.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a741/3782759/56f04833b992/BMRI2013-196409.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a741/3782759/1f4ce02a1169/BMRI2013-196409.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a741/3782759/67902fcc8efe/BMRI2013-196409.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a741/3782759/06d169327f3b/BMRI2013-196409.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a741/3782759/ec39a7608af8/BMRI2013-196409.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a741/3782759/2d9f7325c63d/BMRI2013-196409.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a741/3782759/3299596e5cf2/BMRI2013-196409.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a741/3782759/6da4a519e8fa/BMRI2013-196409.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a741/3782759/1f4af3bfe105/BMRI2013-196409.010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a741/3782759/e44f3665c08d/BMRI2013-196409.011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a741/3782759/cc1396520d5a/BMRI2013-196409.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a741/3782759/56f04833b992/BMRI2013-196409.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a741/3782759/1f4ce02a1169/BMRI2013-196409.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a741/3782759/67902fcc8efe/BMRI2013-196409.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a741/3782759/06d169327f3b/BMRI2013-196409.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a741/3782759/ec39a7608af8/BMRI2013-196409.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a741/3782759/2d9f7325c63d/BMRI2013-196409.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a741/3782759/3299596e5cf2/BMRI2013-196409.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a741/3782759/6da4a519e8fa/BMRI2013-196409.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a741/3782759/1f4af3bfe105/BMRI2013-196409.010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a741/3782759/e44f3665c08d/BMRI2013-196409.011.jpg

相似文献

1
Application of universal stress proteins in probing the dynamics of potent degraders in complex terephthalate metagenome.普遍应激蛋白在探测复杂对苯二甲酸宏基因组中有效降解物的动态变化中的应用。
Biomed Res Int. 2013;2013:196409. doi: 10.1155/2013/196409. Epub 2013 Sep 10.
2
Towards a balanced view of the bacterial tree of life.走向平衡的细菌生命之树观。
Microbiome. 2017 Oct 17;5(1):140. doi: 10.1186/s40168-017-0360-9.
3
Metagenomics--the key to the uncultured microbes.宏基因组学——未培养微生物的关键
Curr Opin Microbiol. 2004 Oct;7(5):492-8. doi: 10.1016/j.mib.2004.08.002.
4
Taxonomic and functional annotation of gut bacterial communities of Eisenia foetida and Perionyx excavatus.鉴定和功能注释食蚯蚓红蚯蚓和中国对虾肠道细菌群落。
Microbiol Res. 2015 Jun;175:48-56. doi: 10.1016/j.micres.2015.03.003. Epub 2015 Mar 10.
5
Influence of imidacloprid on bacterial community diversity of mango orchard soil assessed through 16S rRNA sequencing-based metagenomic analysis.基于 16S rRNA 测序的宏基因组分析评估吡虫啉对芒果园土壤细菌群落多样性的影响。
Environ Monit Assess. 2021 Jan 30;193(2):102. doi: 10.1007/s10661-021-08885-7.
6
Identification and isolation of anaerobic, syntrophic phthalate isomer-degrading microbes from methanogenic sludges treating wastewater from terephthalate manufacturing.从处理对苯二甲酸制造废水的产甲烷污泥中鉴定和分离厌氧、互营性邻苯二甲酸异构体降解微生物。
Appl Environ Microbiol. 2004 Mar;70(3):1617-26. doi: 10.1128/AEM.70.3.1617-1626.2004.
7
Marker genes as predictors of shared genomic function.标记基因作为共享基因组功能的预测因子。
BMC Genomics. 2019 Apr 4;20(1):268. doi: 10.1186/s12864-019-5641-1.
8
MinION™ nanopore sequencing of environmental metagenomes: a synthetic approach.环境宏基因组的MinION™纳米孔测序：一种合成方法。
Gigascience. 2017 Mar 1;6(3):1-10. doi: 10.1093/gigascience/gix007.
9
Multiple syntrophic interactions in a terephthalate-degrading methanogenic consortium.在一个可降解对苯二甲酸的产甲烷共生物中存在多种共生关系。
ISME J. 2011 Jan;5(1):122-30. doi: 10.1038/ismej.2010.125. Epub 2010 Aug 5.
10
Census of the viral metagenome within an activated sludge microbial assemblage.活性污泥微生物组合内病毒宏基因组的普查。
Appl Environ Microbiol. 2010 Apr;76(8):2673-7. doi: 10.1128/AEM.02520-09. Epub 2010 Feb 12.

引用本文的文献

1
Metabolic behavior for a mutant strain with high resistance to ethanol to survive under oenological multi-stress conditions.一种对乙醇具有高抗性的突变菌株在酿酒多胁迫条件下存活的代谢行为。
Front Microbiol. 2023 Mar 9;14:1100501. doi: 10.3389/fmicb.2023.1100501. eCollection 2023.

本文引用的文献

1
Effect of temperature on the release of intentionally and non-intentionally added substances from polyethylene terephthalate (PET) bottles into water: chemical analysis and potential toxicity.温度对聚对苯二甲酸乙二醇酯（PET）瓶中有意和无意添加物质向水中释放的影响：化学分析和潜在毒性。
Food Chem. 2013 Aug 15;139(1-4):672-80. doi: 10.1016/j.foodchem.2013.01.046. Epub 2013 Jan 29.
2
Reconstitution of the cellular response to DNA damage in vitro using damage-activated extracts from mammalian cells.使用哺乳动物细胞中损伤激活的提取物在体外重建细胞对 DNA 损伤的反应。
Exp Cell Res. 2012 Mar 10;318(5):527-38. doi: 10.1016/j.yexcr.2011.12.007. Epub 2011 Dec 16.
3
Developmental Regulation of Genes Encoding Universal Stress Proteins in Schistosoma mansoni.
曼氏血吸虫中编码通用应激蛋白的基因的发育调控
Gene Regul Syst Bio. 2011;5:61-74. doi: 10.4137/GRSB.S7491. Epub 2011 Sep 19.
4
Activity and transcriptional regulation of bacterial protein-like glycerol-3-phosphate dehydrogenase of the haloarchaea in Haloferax volcanii.嗜盐菌古菌 Haloferax volcanii 中细菌样甘油-3-磷酸脱氢酶的活性和转录调控。
J Bacteriol. 2011 Sep;193(17):4469-76. doi: 10.1128/JB.00276-11. Epub 2011 Jul 1.
5
The Vibrio Cholerae Type VI Secretion System: Evaluating its Role in the Human Disease Cholera.霍乱弧菌VI型分泌系统：评估其在人类霍乱疾病中的作用。
Front Microbiol. 2010 Nov 15;1:117. doi: 10.3389/fmicb.2010.00117. eCollection 2010.
6
Effects of salinities on the gene expression of a (NAD+)-dependent glycerol-3-phosphate dehydrogenase in Dunaliella salina.盐度对盐生杜氏藻中依赖 NAD+的甘油-3-磷酸脱氢酶基因表达的影响。
Sci Total Environ. 2011 Mar 1;409(7):1291-7. doi: 10.1016/j.scitotenv.2010.12.038. Epub 2011 Jan 26.
7
Controlling biofilm formation, prophage excision and cell death by rewiring global regulator H-NS of Escherichia coli.通过重新布线大肠杆菌全局调控因子 H-NS 来控制生物膜形成、噬菌体切除和细胞死亡。
Microb Biotechnol. 2010 May;3(3):344-56. doi: 10.1111/j.1751-7915.2010.00164.x. Epub 2010 Mar 4.
8
Genomic organization, structure, regulation and pathogenic role of pilus constituents in major pathogenic Streptococci and Enterococci.主要致病性链球菌和肠球菌中菌毛成分的基因组组织、结构、调控及其致病作用。
Int J Med Microbiol. 2011 Mar;301(3):240-51. doi: 10.1016/j.ijmm.2010.09.003. Epub 2010 Nov 26.
9
Assessing adhesion, biofilm formation and motility of Acidovorax citrulli using microfluidic flow chambers.采用微流控流动室评估酸噬果胶杆菌的黏附、生物膜形成和运动能力。
FEMS Microbiol Lett. 2010 Nov;312(1):33-9. doi: 10.1111/j.1574-6968.2010.02094.x. Epub 2010 Aug 27.
10
Phylogenomics reveals an anomalous distribution of USP genes in metazoans.系统发生基因组学揭示后生动物 USP 基因的异常分布。
Mol Biol Evol. 2011 Jan;28(1):153-61. doi: 10.1093/molbev/msq183. Epub 2010 Jul 21.