一种使用新型微流控装置的灵敏趋化性分析方法。

Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA ; Department of Hepatobiliary Surgery, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, Hubei, China.

Biomed Res Int. 2013;2013:373569. doi: 10.1155/2013/373569. Epub 2013 Sep 14.

Existing chemotaxis assays do not generate stable chemotactic gradients and thus--over time--functionally measure only nonspecific random motion (chemokinesis). In comparison, microfluidic technology has the capacity to generate a tightly controlled microenvironment that can be stably maintained for extended periods of time and is, therefore, amenable to adaptation for assaying chemotaxis. We describe here a novel microfluidic device for sensitive assay of cellular migration and show its application for evaluating the chemotaxis of smooth muscle cells in a chemokine gradient.

现有的趋化性分析方法无法产生稳定的趋化梯度，因此，随着时间的推移，这些方法只能测量非特异性随机运动（趋化运动）。相比之下，微流控技术具有产生可长时间稳定维持的严格受控微环境的能力，因此，适用于趋化性分析。我们在这里描述了一种用于灵敏测定细胞迁移的新型微流控装置，并展示了其在评估趋化因子梯度中平滑肌细胞趋化性的应用。