Department of Urology, Lukas Hospital, Preußenstraße 84, 41464 Neuss, Germany ; West German Cancer Center (WTZ), University of Essen, Essen, Germany ; German Centre for Assessment and Evaluation of Innovative Techniques in Medicine (DZITM), Germany.
Biomed Res Int. 2013;2013:536814. doi: 10.1155/2013/536814. Epub 2013 Sep 17.
To investigate mesh coating modalities with autologous blood components in a recently developed in vitro test system for biocompatibility assessment of alloplastic materials.
Seven different mesh types, currently used in various indications, were randomly investigated. Meshes were coated prior to cultivation with autologous peripheral blood mononuclear cells (PBMCs), platelets, and blood plasma. Pretreated meshes were incubated over 6 weeks in a minced tissue assay, representative for fibroblasts, muscle cells, and endothelial cells originating from 10 different patients. Adherence of those tissues on the meshes was microscopically investigated and semiquantitatively assessed using a previously described scoring system.
Coating with peripheral blood mononuclear cells did not affect the adherence score, whereas coating with platelets and blood plasma increased the score suggesting improved biocompatibility in vitro. The previous ranking of native meshes remained consistent after coating.
Plasma coating of meshes improves their biocompatibility score in a novel in vitro test system.
在最近开发的用于评估同种异体材料生物相容性的体外测试系统中，研究使用自体血液成分进行网片涂层的方式。
本研究随机调查了七种不同类型的网片，这些网片目前被用于不同的适应证。将外周血单个核细胞（PBMCs）、血小板和血浆用于对网片进行预处理后，再将其进行培养。预处理后的网片在一个由 10 位不同患者来源的成纤维细胞、肌肉细胞和内皮细胞的碎组织测定中孵育 6 周。用显微镜观察这些组织在网片上的黏附情况，并使用之前描述的评分系统进行半定量评估。
用外周血单个核细胞进行涂层不会影响黏附评分，而用血小板和血浆进行涂层则会提高评分，提示体外生物相容性得到改善。涂层后，天然网片的先前排名保持一致。
在新型的体外测试系统中，对网片进行血浆涂层可提高其生物相容性评分。
