Institute of Nutrition, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand.
Biomed Res Int. 2013;2013:958567. doi: 10.1155/2013/958567. Epub 2013 Sep 17.
Eryngium foetidum (EF) has long been used as a medicinal plant and culinary spice in tropical regions. Phytochemicals in its leaves have been proposed to be responsible for the anti-inflammatory and antioxidant activities. The present study used in vitro digestion coupled with Caco-2 cells to assess such activities. Caco-2 cells were incubated with aqueous fraction from simulated digestion (bioaccessible fraction) of EF leaves with/without bile extract prior to stimulation with interleukin-1 beta (IL-1β). Monocyte chemoattractant protein-1 (MCP-1) and IL-8 in culture media and the intracellular reactive oxygen species (ROS) were measured. Approximately 24% β-carotene and 35% lutein of leaves were present in the aqueous fraction. The transfer of caffeic and chlorogenic acids to the aqueous fraction was 76%-81%, while that of kaempferol was 48%. Prior incubation of Caco-2 cells with the bioaccessible fraction suppressed IL-1β activated IL-8 and MCP-1 by 33%, but the fraction lacking mixed micelles decreased IL-8 and MCP-1 levels only by 11%. The pretreatment of Caco-2 cells with the bioaccessible fraction of EF reduced ROS by 34%; the fraction lacking mixed micelles decreased ROS by 28%. These data suggest that bioactive compounds partitioning in mixed micelles play a significant role to suppress the proinflammatory insult but with a modest antioxidant effect.
臭刺（EF）长期以来一直被用作热带地区的药用植物和烹饪香料。其叶子中的植物化学物质被认为是其抗炎和抗氧化活性的原因。本研究使用体外消化法结合 Caco-2 细胞来评估这些活性。Caco-2 细胞在用/不用胆汁提取物孵育模拟消化（可生物利用的部分）EF 叶后，用白细胞介素-1β（IL-1β）刺激。测量培养基中单核细胞趋化蛋白-1（MCP-1）和白细胞介素-8（IL-8）以及细胞内活性氧（ROS）的含量。叶片中的β-胡萝卜素和叶黄素分别约有 24%和 35%存在于水相中。咖啡酸和绿原酸向水相的转移率为 76%-81%，而山奈酚的转移率为 48%。Caco-2 细胞先用可生物利用的部分孵育，可抑制 IL-1β 激活的 IL-8 和 MCP-1 达 33%，但缺乏混合胶束的部分仅降低 IL-8 和 MCP-1 水平 11%。EF 的可生物利用部分预处理 Caco-2 细胞可使 ROS 降低 34%；缺乏混合胶束的部分使 ROS 降低 28%。这些数据表明，分配在混合胶束中的生物活性化合物在抑制炎症侵袭方面起着重要作用，但抗氧化作用适中。
