Department of Microbiology and Immunology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, People's Republic of China.
Diagn Pathol. 2013 Oct 24;8:179. doi: 10.1186/1746-1596-8-179.
Astragalus memebranaceus is a traditional Chinese herbal medicine used in treatment of common cold, diarrhea, fatigue, anorexia and cardiac diseases. Recently, there are growing evidences that Astragalus extract may be a potential anti-tumorigenic agent. Some research showed that the total saponins obtained from Astragalus membranaceus possess significant antitumorigenic activity. Gastric cancer is one of the most frequent cancers in the world, almost two-thirds of gastric cancer cases and deaths occur in less developed regions. But the effect of Astragalus membranaceus on proliferation, invasion and apoptosis of gastric cancer BGC-823 cells remains unclear.
Astragalus saponins were extracted. Cells proliferation was determined by CCK-8 assay. Cell cycle and apoptosis were detected by the flow cytometry. Boyden chamber was used to evaluate the invasion and metastasis capabilities of BGC-823 cells. Tumor growth was assessed by subcutaneous inoculation of cells into BALB/c nude mice.
The results demonstrated that total Astragalus saponins could inhibit human gastric cancer cell growth both in vitro and in vivo, in additional, Astragalus saponins deceased the invasion ability and induced the apoptosis of gastric cancer BGC-823 cells.
Total Astragalus saponins inhibited human gastric cancer cell growth, decreased the invasion ability and induced the apoptosis. This suggested the possibility of further developing Astragalus as an alternative treatment option, or perhaps using it as adjuvant chemotherapeutic agent in gastric cancer therapy.
黄芪是一种传统的中草药，用于治疗普通感冒、腹泻、疲劳、食欲不振和心脏病。最近，越来越多的证据表明，黄芪提取物可能是一种潜在的抗肿瘤药物。一些研究表明，从黄芪中提取的总皂苷具有显著的抗肿瘤活性。胃癌是世界上最常见的癌症之一，几乎三分之二的胃癌病例和死亡发生在欠发达地区。但是黄芪对胃癌 BGC-823 细胞增殖、侵袭和凋亡的影响尚不清楚。
提取黄芪皂苷。用 CCK-8 法测定细胞增殖。用流式细胞术检测细胞周期和凋亡。Boyden 室用于评估 BGC-823 细胞的侵袭和转移能力。通过皮下接种细胞到 BALB/c 裸鼠来评估肿瘤生长。
结果表明，黄芪总皂苷既能体外又能体内抑制人胃癌细胞的生长，此外，黄芪皂苷能降低胃癌 BGC-823 细胞的侵袭能力并诱导其凋亡。
黄芪总皂苷抑制人胃癌细胞的生长，降低侵袭能力并诱导凋亡。这表明进一步开发黄芪作为替代治疗选择的可能性，或者将其用作胃癌治疗的辅助化疗药物的可能性。
