van Muijen G N, Ruiter D J, Franke W W, Achtstätter T, Haasnoot W H, Ponec M, Warnaar S O
Exp Cell Res. 1986 Jan;162(1):97-113. doi: 10.1016/0014-4827(86)90429-5.
Three monoclonal antibodies, 1C7, 2D7 and 6B10, directed against cytokeratins of human esophagus were isolated and characterized by one- and two-dimensional gel electrophoresis and by immunohistochemical staining on sections of human epithelial tissues. In immunoblot experiments, antibodies of clones 1C7 (IgG2a) and 2D7 (IgG2b) react only with cytokeratin no. 13 of the acidic (type I) subfamily of cytokeratin polypeptides (Mr 54000; pI 5.1); antibodies of clone 6B10 (IgG1) detect only cytokeratin no. 4 (Mr 59000; pI 7.3) of the basic (type II) cytokeratin subfamily and allows the detection of this protein and possible degradation products at high sensitivity. In immunohistochemical staining all three antibodies stain non-cornifying squamous epithelium (e.g., tongue, esophagus, anus) and transitional epithelium of the bladder. Antibodies of clone 6B10 also stain cells in certain ciliated pseudostratified epithelia and ductal epithelia of various exocrine glands. These monoclonal antibodies are the first examples of antibodies specific for individual cytokeratin polypeptides characteristic of certain complex epithelia. They allow the identification of distinct minor populations of cells present in certain complex and glandular epithelia and in tumors derived therefrom which hitherto have not been distinguished. The possible reasons for the occurrence of cell type heterogeneity of cytokeratin expression in complex epithelia and in some carcinomas are discussed.
分离出三种针对人食管细胞角蛋白的单克隆抗体1C7、2D7和6B10，并通过一维及二维凝胶电泳以及对人上皮组织切片进行免疫组织化学染色对其进行了表征。在免疫印迹实验中，克隆1C7（IgG2a）和2D7（IgG2b）的抗体仅与细胞角蛋白酸性（I型）亚家族的细胞角蛋白13发生反应（分子量54000；等电点5.1）；克隆6B10（IgG1）的抗体仅检测碱性（II型）细胞角蛋白亚家族的细胞角蛋白4（分子量59000；等电点7.3），并能够高灵敏度地检测该蛋白及其可能的降解产物。在免疫组织化学染色中，所有三种抗体均能对非角化鳞状上皮（如舌、食管、肛门）和膀胱的移行上皮进行染色。克隆6B10的抗体还能对某些纤毛假复层上皮以及各种外分泌腺的导管上皮中的细胞进行染色。这些单克隆抗体是针对某些复杂上皮所特有的单个细胞角蛋白多肽的特异性抗体的首例。它们能够识别某些复杂和腺上皮及其衍生肿瘤中存在的迄今尚未区分的不同小细胞群体。文中还讨论了复杂上皮和某些癌中细胞角蛋白表达出现细胞类型异质性的可能原因。
