Department of Pharmaceutical Biology, Institute of Pharmacy, Ernst-Moritz-Arndt University, Friedrich-Ludwig-Jahn Str. 15a, 17487, Greifswald, Germany; ZIK Plasmatis, Leibniz Institute for Plasma Sciences and Technology e.V. (INP), Felix-Hausdorff-Str. 2, 17489, Greifswald, Germany.
Cell Biol Int. 2014 Apr;38(4):412-25. doi: 10.1002/cbin.10200. Epub 2014 Feb 10.
Modern non-thermal atmospheric pressure plasma sources enable controllable interaction with biological systems. Their future applications - e.g. wound management - are based on their unique mixture of reactive components sparking both stimulatory as well as inhibitory processes. To gain detailed understanding of plasma-cell interaction and with respect to risk awareness, key mechanisms need to be identified. This study focuses on the impact of an argon non-thermal atmospheric pressure plasma jet (kINPen 09) on human HaCaT keratinocytes. With increasing duration, cell viability decreased. In accordance, cells accumulated in G2/M phase within the following 24 h. DNA single-strand breaks were detected immediately after treatment and receded in the aftermath, returning to control levels after 24 h. No directly plasma-related DNA double-strand breaks were detected over the same time. Concurrently, DNA synthesis decreased. Coincident with treatment time, an increase in intracellular 2',7'-dichlorodihydrofluorescein diacetate (H(2)DCFDA) conversion increased reactive oxygen species (ROS) levels. The radical scavenging activity of culture medium crucially influenced these effects. Thus, ROS changed DNA integrity, and the effectiveness of cellular defence mechanisms characterises the interaction of non-thermal plasma and eukaryotic cells. Effects were time-dependent, indicating an active response of the eukaryotic cells. Hence, a stimulation of eukaryotic cells using short-term non-thermal plasma treatment seems possible, eg in the context of chronic wound care. Long-term plasma treatments stopped in cell proliferation and apoptosis, which might be relevant in controlling neoplastic conditions.
现代非热大气压等离子体源能够实现与生物系统的可控相互作用。它们未来的应用——例如伤口管理——基于其独特的反应成分混合物，激发了刺激和抑制过程。为了详细了解等离子体与细胞的相互作用，并提高对风险的认识，需要确定关键机制。本研究聚焦于氩气非热大气压等离子体射流（kINPen 09）对人 HaCaT 角质形成细胞的影响。随着作用时间的增加，细胞活力降低。相应地，细胞在接下来的 24 小时内积累在 G2/M 期。处理后立即检测到 DNA 单链断裂，并在随后消退，24 小时后恢复到对照水平。在同一时间内没有检测到直接与等离子体相关的 DNA 双链断裂。同时，DNA 合成减少。与处理时间同时，细胞内 2'，7'-二氯二氢荧光素二乙酸酯（H（2）DCFDA）转化的增加增加了活性氧（ROS）水平。培养基的自由基清除活性对这些影响至关重要。因此，ROS 改变了 DNA 的完整性，而细胞防御机制的有效性则是描述非热等离子体与真核细胞相互作用的特征。这些效应具有时间依赖性，表明真核细胞的主动反应。因此，使用短期非热等离子体处理刺激真核细胞似乎是可能的，例如在慢性伤口护理的情况下。长期等离子体处理停止了细胞增殖和凋亡，这可能与控制肿瘤条件有关。
