Department of Applied Chemistry and Life Science, Toyohashi University of Technology, Toyohashi, Japan.
PLoS One. 2020 May 6;15(5):e0232724. doi: 10.1371/journal.pone.0232724. eCollection 2020.
DNA damage in the A549 human lung cancer cell line treated with cold plasma irradiation was investigated. We confirmed that cold atmospheric plasma generated reactive oxygen and nitrogen species (RONS) in a liquid, and the intracellular RONS level was increased in plasma-irradiated cells. However, a notable decrease in cell viability was not observed 24 hours after plasma irradiation. Because RONS induce oxidative damage in cells, strand breaks and chemical modification of DNA in the cancer cells were investigated. We found that 8-oxoguanine (8-oxoG) formation as well as DNA strand breaks, which have been thoroughly investigated, were induced by plasma irradiation. In addition, up-regulation of 8-oxoG repair enzyme was observed after plasma irradiation.
研究了冷等离子体照射对 A549 人肺癌细胞系的 DNA 损伤。我们证实，冷大气压等离子体在液体中产生活性氧和氮物质（RONS），并且细胞内的 RONS 水平在等离子体照射的细胞中增加。然而，在等离子体照射后 24 小时，细胞活力并没有明显下降。由于 RONS 会在细胞中引起氧化损伤，因此研究了癌细胞中的 DNA 链断裂和化学修饰。我们发现，8-氧鸟嘌呤（8-oxoG）的形成以及已经深入研究的 DNA 链断裂是由等离子体照射诱导的。此外，在等离子体照射后观察到 8-氧鸟嘌呤修复酶的上调。
