乙酰胆碱酯酶催化的水解反应可实现对病原体的超灵敏肉眼检测。
Acetylcholinesterase-catalyzed hydrolysis allows ultrasensitive detection of pathogens with the naked eye.
作者信息
Liu Dingbin, Wang Zhantong, Jin Albert, Huang Xinglu, Sun Xiaolian, Wang Fu, Yan Qiang, Ge Shengxiang, Xia Ningshao, Niu Gang, Liu Gang, Hight Walker A R, Chen Xiaoyuan
机构信息
Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892 (USA).
出版信息
Angew Chem Int Ed Engl. 2013 Dec 23;52(52):14065-9. doi: 10.1002/anie.201307952. Epub 2013 Oct 23.
Abstract
This paper describes a rapid diagnostic platform for pathogen detection based on the acetylcholinesterase-catalyzed hydrolysis reaction. Owing to the signal amplification strategies, the sensitivity of this assay is comparable to that of PCR. In addition, the readout of this assay is based on the color change of solutions, which can be easily observed by the naked eye alone.
摘要
本文描述了一种基于乙酰胆碱酯酶催化水解反应的病原体快速诊断平台。由于采用了信号放大策略，该检测方法的灵敏度与聚合酶链反应（PCR）相当。此外，该检测方法的读数基于溶液的颜色变化，仅凭肉眼即可轻松观察到。
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