Liu Dingbin, Chen Xiaoyuan
College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Molecular Recognition and Biosensing, and Collaborative Innovation Center of Chemical Science and Engineering, Nankai University, Tianjin, 300071, China.
Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD, 20892, USA.
Methods Mol Biol. 2017;1530:307-316. doi: 10.1007/978-1-4939-6646-2_18.
Enzyme-linked immunosorbent assay (ELISA) is a well-known strategy for biomarker detection with a color change, which can be seen by the naked eyes. However, the moderate sensitivity of conventional ELISA limits its applications in many cases where the concentrations of biomarker are very low, such as cancer diagnosis. Here we describe an ultrasensitive colorimetric assay based on acetylcholinesterase (AChE)-catalyzed hydrolysis reaction, whose products trigger the aggregation of gold nanoparticles (AuNPs), causing a distinct color change of the solution from red to purple. This enhanced colorimetric immunoassay offers extremely high sensitivity and specificity. In this study, we employed enterovirus 71 (EV71), the major cause of hand, foot, and mouth disease (HFMD), as a model to evaluate the analytical performance of the plasmonic immunoassay.
酶联免疫吸附测定（ELISA）是一种利用颜色变化进行生物标志物检测的知名策略，这种颜色变化肉眼可见。然而，传统ELISA的中等灵敏度限制了其在许多生物标志物浓度非常低的情况下的应用，比如癌症诊断。在此，我们描述了一种基于乙酰胆碱酯酶（AChE）催化水解反应的超灵敏比色测定法，其产物会引发金纳米颗粒（AuNPs）的聚集，导致溶液颜色从红色明显变为紫色。这种增强型比色免疫测定法具有极高的灵敏度和特异性。在本研究中，我们以手足口病（HFMD）的主要病原体肠道病毒71型（EV71）作为模型，评估等离子体免疫测定法的分析性能。
