Krautkrämer Ellen, Grouls Stephan, Hettwer David, Rafat Neysan, Tönshoff Burkhard, Zeier Martin
Department of Nephrology, University of Heidelberg, Heidelberg, Germany.
J Virol. 2014 Jan;88(1):483-9. doi: 10.1128/JVI.02063-13. Epub 2013 Oct 23.
Infections with hemorrhagic fever viruses are characterized by increased permeability leading to capillary leakage. Hantavirus infection is associated with endothelial dysfunction, and the clinical course is related to the degree of vascular injury. Circulating endothelial progenitor cells (cEPCs) play a pivotal role in the repair of the damaged endothelium. Therefore, we analyzed the number of cEPCs and their mobilizing growth factors in patients suffering from hantavirus disease induced by infection with Puumala virus. The numbers of EPCs of 36 hantavirus-infected patients and age- and gender-matched healthy controls were analyzed by flow cytometry. Concentrations of cEPC-mobilizing growth factors in plasma were determined by enzyme-linked immunosorbent assay. Laboratory parameters were correlated with the number of cEPCs. In patients infected with hantavirus, the number of cEPCs was significantly higher than that in healthy controls. Levels of mobilizing cytokines were upregulated in patients, and the mobilization of cEPCs is paralleled with the normalization of clinical parameters. Moreover, higher levels of cEPCs correlated with higher serum albumin levels and platelet concentrations. Our data indicate that cEPCs may play a role in the repair of hantavirus-induced endothelial damage, thereby influencing the clinical course and the severity of symptoms.
出血热病毒感染的特征是通透性增加，导致毛细血管渗漏。汉坦病毒感染与内皮功能障碍有关，临床病程与血管损伤程度相关。循环内皮祖细胞（cEPCs）在受损内皮的修复中起关键作用。因此，我们分析了感染普马拉病毒所致汉坦病毒病患者的cEPCs数量及其动员生长因子。通过流式细胞术分析了36例汉坦病毒感染患者及年龄和性别匹配的健康对照者的内皮祖细胞数量。采用酶联免疫吸附测定法测定血浆中cEPCs动员生长因子的浓度。实验室参数与cEPCs数量相关。在汉坦病毒感染患者中，cEPCs数量显著高于健康对照者。患者体内动员细胞因子水平上调，cEPCs的动员与临床参数的正常化平行。此外，较高水平的cEPCs与较高的血清白蛋白水平和血小板浓度相关。我们的数据表明，cEPCs可能在汉坦病毒诱导的内皮损伤修复中发挥作用，从而影响临床病程和症状严重程度。
