Liu Mengyang, Schmitner Nicole, Sandrian Michelle G, Zabihian Behrooz, Hermann Boris, Salvenmoser Willi, Meyer Dirk, Drexler Wolfgang
Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Währinger Gürtel 18-20, AKH 4L, 1090 Vienna, Austria.
Biomed Opt Express. 2013 Aug 29;4(10):1846-55. doi: 10.1364/BOE.4.001846. eCollection 2013.
For the first time the far red fluorescent protein (FP) E2-Crimson genetically expressed in the exocrine pancreas of adult zebrafish has been non-invasively mapped in 3D in vivo using photoacoustic tomography (PAT). The distribution of E2-Crimson in the exocrine pancreas acquired by PAT was confirmed using epifluorescence imaging and histology, with optical coherence tomography (OCT) providing complementary structural information. This work demonstrates the depth advantage of PAT to resolve FP in an animal model and establishes the value of E2-Crimson for PAT studies of transgenic models, laying the foundation for future longitudinal studies of the zebrafish as a model of diseases affecting inner organs.
首次利用光声层析成像（PAT）在体内对成年斑马鱼外分泌胰腺中基因表达的远红荧光蛋白（FP）E2-深红色进行了三维无创成像。通过落射荧光成像和组织学方法证实了PAT获取的外分泌胰腺中E2-深红色的分布，并利用光学相干断层扫描（OCT）提供了补充结构信息。这项工作证明了PAT在动物模型中解析FP的深度优势，并确立了E2-深红色在转基因模型PAT研究中的价值，为未来将斑马鱼作为影响内脏器官疾病模型的纵向研究奠定了基础。
