Ulusoy Şeyma, İnal Ebrar, Küpeli Akkol Esra, Çiçek Mahmut, Kartal Murat, Sobarzo-Sánchez Eduardo
Department of Pharmacognosy and Natural Products Chemistry, Institute of Health Sciences, Bezmialem Vakif University, Istanbul, Türkiye.
Department of Pharmacognosy, Faculty of Pharmacy, Bezmialem Vakif University, Istanbul, Türkiye.
Front Pharmacol. 2024 Jul 11;15:1410854. doi: 10.3389/fphar.2024.1410854. eCollection 2024.
The aim of this study was to investigate the effects of L. and L. plants on obesity . Extracts were prepared from leaves, flowers, fruits and from leaves, flowers, and fruits using 100% water and 70% ethanol. The total phenol and flavonoid contents of the extracts were quantified spectrophotometrically. The findings revealed that the ethanol extracts of and flowers had the highest phenolic content, while the ethanol extracts of flowers and leaves had the highest flavonoid content. Qualification and quantification of the phenolic contents of the extracts were carried out using liquid chromatography-high resolution mass spectrometry (LC-HRMS) analyses. The study investigated the effects of various extracts on plasma levels of leptin, insulin, triiodothyronine (T), thyroxine (T), triglycerides, high-density lipoprotein (HDL), low-density lipoprotein (LDL) and lipase enzyme in obesity-induced rats. The results showed that the ethanol extract of flowers, as well as the ethanol and water extracts of leaves, resulted in body weight reduction in rats with obesity. Additionally, these extracts were found to decrease serum levels of LDL, triglycerides, leptin, lipase, TNF-α, and IL-1β while increasing levels of HDL and adiponectin. The LC-HRMS results demonstrated that all three extracts exhibited relatively high concentrations of luteolin-7-glycoside and kaempferol, in comparison to the other extracts. The ethanol extract of flowers contained 653.04 mg/100 g of luteolin-7-glycoside and 62.63 mg/100 g of kaempferol. The ethanol extract of leaves contained 1,720.26 mg/100 g of luteolin-7-glycoside and 95.85 mg/100 g of kaempferol. The water extract of leaves contained 690.49 mg/100 g of luteolin-7-glycoside and 194.41 mg/100 g of kaempferol. The study suggests that the ethanol extract of flowers and leaves, as well as the water extract of leaves, may have potential benefits in treating obesity. However, further controlled clinical studies are necessary to evaluate the clinical efficacy of in treating obesity and investigate the anti-obesogenic effects of luteolin-7-glycoside and kaempferol separately, both in their pure form and in combination.
本研究的目的是调查[两种植物名称未明确给出，暂用L.和L.]植物对肥胖的影响。使用100%水和70%乙醇从[两种植物名称未明确给出，暂用L.]的叶子、花朵、果实以及[两种植物名称未明确给出，暂用L.]的叶子、花朵和果实中制备提取物。采用分光光度法定量提取物中的总酚和黄酮含量。研究结果显示，[两种植物名称未明确给出，暂用L.]花朵的乙醇提取物酚含量最高，而[两种植物名称未明确给出，暂用L.]花朵和[两种植物名称未明确给出，暂用L.]叶子的乙醇提取物黄酮含量最高。使用液相色谱 - 高分辨率质谱（LC - HRMS）分析对提取物中的酚类成分进行定性和定量。该研究调查了各种提取物对肥胖诱导大鼠血浆中瘦素、胰岛素、三碘甲状腺原氨酸（T3）、甲状腺素（T4）、甘油三酯、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）和脂肪酶水平的影响。结果表明，[两种植物名称未明确给出，暂用L.]花朵的乙醇提取物以及[两种植物名称未明确给出，暂用L.]叶子的乙醇和水提取物可使肥胖大鼠体重减轻。此外，发现这些提取物可降低血清中LDL、甘油三酯、瘦素、脂肪酶、TNF - α和IL - 1β的水平，同时提高HDL和脂联素的水平。LC - HRMS结果表明，与其他提取物相比，这三种提取物均表现出相对较高浓度的木犀草素 - 7 - 糖苷和山奈酚。[两种植物名称未明确给出，暂用L.]花朵的乙醇提取物含有653.04 mg/100 g木犀草素 - 7 - 糖苷和62.63 mg/100 g山奈酚。[两种植物名称未明确给出，暂用L.]叶子的乙醇提取物含有1720.26 mg/100 g木犀草素 - 7 - 糖苷和95.85 mg/100 g山奈酚。[两种植物名称未明确给出，暂用L.]叶子的水提取物含有690.49 mg/100 g木犀草素 - 7 - 糖苷和194.41 mg/100 g山奈酚。该研究表明，[两种植物名称未明确给出，暂用L.]花朵和叶子的乙醇提取物以及[两种植物名称未明确给出，暂用L.]叶子的水提取物在治疗肥胖方面可能具有潜在益处。然而，需要进一步的对照临床研究来评估[两种植物名称未明确给出，暂用L.]治疗肥胖的临床疗效，并分别研究木犀草素 - 7 - 糖苷和山奈酚以纯形式及其组合形式的抗肥胖作用。
