von Rheinbaben F, Stitz L, Rott R
J Gen Virol. 1985 Dec;66 ( Pt 12):2777-80. doi: 10.1099/0022-1317-66-12-2777.
The effect of interferon (IFN) on infection and maintenance of persistent infection of Borna disease (BD) virus in cell cultures was investigated. Acutely BD virus-infected primary rabbit brain and rat lung cells produced significant levels of interferon detectable 3 days post-infection in the culture supernatants. Rat brain and rat lung cells persistently infected with BD virus produced only moderate levels of IFN over a long period. In contrast, persistently infected Madin-Darby canine kidney (MDCK) cells did not produce detectable amounts of IFN. Exogenous homologous IFN completely inhibited the expression of BD virus antigen in acutely infected rabbit brain cells, when added during the first 24 h after infection. IFN added later (2 to 6 days post-infection) reduced virus titres to different degrees depending on the onset of treatment. However, IFN added to persistently infected rat lung cells did not appear to influence the degree or quality of BD virus antigen expression or the intracellular amount of infectious virus. Two facts indicate that IFN is not involved in the establishment or maintenance of persistent BD virus infection in vitro. Thus, MDCK cells, which could not be induced to produce IFN, can be readily persistently infected with BD virus in vitro, and exogenous IFN did not appear to influence persistent BD virus infection.
研究了干扰素（IFN）对博尔纳病（BD）病毒在细胞培养物中感染及持续感染维持的影响。急性感染BD病毒的原代兔脑和大鼠肺细胞在感染后3天，培养上清液中可检测到显著水平的干扰素。长期持续感染BD病毒的大鼠脑和大鼠肺细胞仅产生中等水平的IFN。相比之下，持续感染的Madin-Darby犬肾（MDCK）细胞未产生可检测量的IFN。在感染后的最初24小时内添加外源性同源IFN，可完全抑制急性感染兔脑细胞中BD病毒抗原的表达。感染后期（感染后2至6天）添加IFN，根据治疗开始时间不同程度地降低病毒滴度。然而，添加到持续感染大鼠肺细胞中的IFN似乎并未影响BD病毒抗原表达的程度或质量，也未影响细胞内感染性病毒的量。有两个事实表明IFN不参与体外BD病毒持续感染的建立或维持。因此，无法诱导产生IFN的MDCK细胞在体外可轻易被BD病毒持续感染，且外源性IFN似乎并未影响BD病毒的持续感染。
