School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China,
Antonie Van Leeuwenhoek. 2014 Jan;105(1):135-41. doi: 10.1007/s10482-013-0061-8. Epub 2013 Oct 25.
Three halophilic archaeal strains, XD46(T), YJ-63-S1 and ZS-1-H, were isolated from three Chinese marine solar salterns. All were observed to have pleomorphic cells that lysed in distilled water, stained Gram-negative and formed red-pigmented colonies. They were found to grow optimally at 37 °C, at pH 7.0 and in the presence of 2.6 M NaCl and 0.05 M Mg(2+). The major polar lipids were identified as those typical for members of the Halobacteriaceae but also included major glycolipids chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1), mannosyl glucosyl diether (DGD-1) and two unidentified ones. The 16S rRNA gene sequences of the three strains were 99.8-100 % identical, showing most similarity to sequences of members of the family Halobacteriaceae, and clustering together as a distinct clade in phylogenetic tree reconstructions. The rpoB' gene similarities between the three strains were 98.7-100 % and lower to the sequences of other halobacteria. Their DNA G+C contents were determined to be 65.1-65.5 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strains XD46(T) (=CGMCC 1.12237(T) = JCM 18649(T)), YJ-63-S1 (=CGMCC 1.12574) and ZS-1-H (=CGMCC 1.12544) represent a novel species in a new genus within the family Halobacteriaceae, for which the name Salinarubrum litoreum gen. nov., sp. nov. is proposed.
从中国三个海洋晒盐场中分离到三株嗜盐古菌 XD46(T)、YJ-63-S1 和 ZS-1-H。所有菌株均观察到具有多形性细胞，在蒸馏水中溶解，革兰氏染色阴性，形成红色色素菌落。它们在 37°C、pH 值 7.0、2.6 M NaCl 和 0.05 M Mg(2+)的条件下生长最佳。主要极性脂类被鉴定为典型的盐杆菌科成员，但也包括主要的糖脂，与硫酸甘露糖葡糖基二醚（S-DGD-1）、甘露糖葡糖基二醚（DGD-1）和两种未鉴定的糖脂在色谱上相同。三株菌的 16S rRNA 基因序列相似度为 99.8-100%，与盐杆菌科成员的序列最相似，并在系统发育树重建中聚为一个独特的分支。三株菌的 rpoB'基因相似度为 98.7-100%，与其他嗜盐菌的序列相似度较低。它们的 DNA G+C 含量为 65.1-65.5 mol%。表型、化学分类和系统发育特性表明，XD46(T) 株（=CGMCC 1.12237(T)=JCM 18649(T)）、YJ-63-S1 株（=CGMCC 1.12574）和 ZS-1-H 株（=CGMCC 1.12544）代表盐杆菌科一个新属中的一个新种，建议将其命名为 Salinarubrum litoreum 属，新种。
